Cao Yufeng, Zhu Lisai, Liu Dan, Guo Changming, Yuan Yue, Shi Chengbo, Liu Zhaohui, Wang Xinping
College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, Jilin, China.
Changchun Institute of Biological Products Co. Ltd., 3456 Xian Road, Changchun, 130062, Jilin, China.
Virus Genes. 2017 Jun;53(3):483-486. doi: 10.1007/s11262-017-1435-1. Epub 2017 Feb 15.
Hepatitis E virus (HEV) infection is an emerging disease with zoonotic transmission that represents a serious public health concern, especially in developing countries. Here we characterize a novel HEV strain CCST-517, which possesses a complete genome sequence of 7284 bp with typical HEV genome organization including 5' and 3' non-coding regions and three open reading frames. The sequence identities of CCST-517 with known HEV genotype 1, 2, 3, and 4 were 73.4-73.7, 73.2, 80.4-90.4, and 75.1-75.7%, respectively. Phylogenetic analysis clustered CCST-517 to the clade of HEV genotype 3a, together with the Japanese human HEV isolate (HE-JA10) and United States human HEV isolate (HEV-US2). Similarity plot analysis indicated that the fragment extending from 4500 to 5500 nt included evidence of one intra-genotype recombination event in the genome sequence of the CCST-517 strain. To our knowledge, this is the first report of HEV genotype 3a with its complete genome sequence revealed in China. Our findings revealed a close phylogenetic relationship of CCST-517 to human HEV-US2 and HE-JA10, implying cross-species transmission of HEV between pigs and humans.
戊型肝炎病毒（HEV）感染是一种新兴的人畜共患病，是一个严重的公共卫生问题，在发展中国家尤为如此。在此，我们对一株新型HEV毒株CCST-517进行了特征分析，该毒株拥有7284 bp的完整基因组序列，具有典型的HEV基因组结构，包括5'和3'非编码区以及三个开放阅读框。CCST-517与已知的HEV基因型1、2、3和4的序列同一性分别为73.4-73.7%、73.2%、80.4-90.4%和75.1-75.7%。系统发育分析将CCST-517聚类到HEV基因型3a分支，与日本人类HEV分离株（HE-JA10）和美国人类HEV分离株（HEV-US2）在一起。相似性图谱分析表明，从4500到5500 nt延伸的片段包括CCST-517毒株基因组序列中一个基因型内重组事件的证据。据我们所知，这是在中国首次报道具有完整基因组序列的HEV基因型3a。我们的研究结果揭示了CCST-517与人类HEV-US2和HE-JA10之间密切的系统发育关系，这意味着HEV在猪和人之间存在跨物种传播。
