Agricultural & Biological Engineering, Physiological Sensing Facility at the Bindley Bioscience Center & Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA.
Molecular Biosciences, The University of Texas at Austin, 1 University Station A6700, Austin, TX 78712, USA.
Lab Chip. 2017 Mar 14;17(6):1095-1103. doi: 10.1039/c6lc01370h.
This report describes the development of lab-on-a-chip device designed to measure changes in cellular ion gradients that are induced by changes in gravitational (g) forces. The bioCD presented here detects differential calcium ion concentrations outside of individual cells. The device includes sufficient replicates for statistical analysis of the gradients around multiple single cells and around control wells that are empty or include dead cells. In the data presented, the degree of the cellular response correlates with the magnitude of the g-force applied via rotation of the bioCD. The experiments recorded the longest continuous observation of a cellular response to hypergravity made to date, and they demonstrate the potential utility of this device for assaying the threshold of cells' g-force responses in spaceflight conditions.
本报告描述了一种芯片实验室设备的开发，该设备旨在测量细胞离子梯度的变化，这些变化是由重力（g）力的变化引起的。本文介绍的生物芯片能够检测单个细胞外的钙离子浓度差异。该设备包括足够的重复样本，可用于对多个单细胞周围和空的或包含死细胞的对照孔周围的梯度进行统计分析。在呈现的数据中，细胞反应的程度与通过生物芯片的旋转施加的 g 力的大小相关。这些实验记录了迄今为止对细胞对超重力反应的最长连续观察，并且它们展示了该设备在测定细胞在空间飞行条件下对 g 力反应的阈值方面的潜在用途。
