携带d(CG.GC)n、d(CA.GT)n和d(CT.GA)n序列的猿猴病毒40重组体的获得。插入的简单重复序列的稳定性。

The obtention of simian virus 40 recombinants carrying d(CG.GC)n, d(CA.GT)n and d(CT.GA)n sequences. Stability of the inserted simple repeating sequences.

作者信息

Casasnovas J M, Ellison M J, Rodriguez-Campos A, Azorin F

机构信息

Centro de Investigacion y Desarrollo, Consejo Superior de Investigaciones Cientificas, Universidad Politecnica de Catalunya, Barcelona, Spain.

出版信息

Eur J Biochem. 1987 Sep 15;167(3):489-92. doi: 10.1111/j.1432-1033.1987.tb13363.x.

DOI:10.1111/j.1432-1033.1987.tb13363.x

PMID:2820728

Abstract

A general strategy for the introduction of simple repeating DNA sequences into the simian virus 40 (SV40) has been developed. SV40 recombinants carrying d(CG.GC)5, d(CA.GT)30 or d(CT.GA)22 insertions at either the TaqI site (position 4739) or the HpaII site (position 346) were obtained and the stability of the inserted DNA sequences studied. The palindromic potentially Z-DNA-forming d(CG.GC)n sequence was found to be highly unstable when compared to either d(CA.GT)n or d(CT.GA)n.

摘要

已开发出一种将简单重复DNA序列引入猿猴病毒40（SV40）的通用策略。获得了在TaqI位点（位置4739）或HpaII位点（位置346）携带d（CG.GC）5、d（CA.GT）30或d（CT.GA）22插入片段的SV40重组体，并研究了插入DNA序列的稳定性。与d（CA.GT）n或d（CT.GA）n相比，发现回文结构的潜在形成Z-DNA的d（CG.GC）n序列高度不稳定。