Laboratory of Organic Chemistry, Wageningen University & Research , Stippeneng 4, 6708 WE Wageningen, The Netherlands.
TI-COAST , Science Park 904, 1098 XH Amsterdam, The Netherlands.
Anal Chem. 2017 Feb 7;89(3):1427-1432. doi: 10.1021/acs.analchem.6b04012. Epub 2017 Jan 26.
A simplified coupling of surface plasmon resonance (SPR) immuno-biosensing with ambient ionization mass spectrometry (MS) was developed. It combines two orthogonal analysis techniques: the biosensing capability of SPR and the chemical identification power of high resolution MS. As a proof-of-principle, deoxynivalenol (DON), an important mycotoxin, was captured using an SPR gold chip containing an antifouling layer and monoclonal antibodies against the toxin and, after washing, the chip could be taken out and analyzed by direct spray MS of the biosensor chip to confirm the identity of DON. Furthermore, cross-reacting conjugates of DON present in a naturally contaminated beer could be successfully identified, thus showing the potential of rapid identification of (un)expected cross-reacting molecules.
建立了一种简单的表面等离子体共振（SPR）免疫生物传感与环境电离质谱（MS）联用方法。它结合了两种正交分析技术：SPR 的生物传感能力和高分辨率 MS 的化学识别能力。作为原理验证，使用含有防污层和针对毒素的单克隆抗体的 SPR 金芯片捕获脱氧雪腐镰刀菌烯醇（DON），一种重要的真菌毒素，然后在清洗后，将芯片取出并用生物传感器芯片的直接喷雾 MS 进行分析，以确认 DON 的身份。此外，还成功鉴定了天然污染啤酒中存在的 DON 交叉反应结合物，从而显示出快速鉴定（未）预期的交叉反应分子的潜力。
