Department of Chemistry, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
Sci Rep. 2017 Nov 23;7(1):16104. doi: 10.1038/s41598-017-16494-z.
This study demonstrates the quantitative capabilities of coated blade spray (CBS) mass spectrometry (MS) for the concomitant analysis of multiple target substances in biofluid spots. In CBS-MS the analytes present in a given sample are first isolated and enriched in the thin coating of the CBS device. After a quick rinsing of the blade surface, as to remove remaining matrix, the analytes are quickly desorbed with the help of a solvent and then directly electrosprayed into the MS analyzer. Diverse pain management drugs, controlled substances, and therapeutic medications were successfully determined using only 10 µL of biofluid, with limits of quantitation in the low/sub ng·mL level attained within 7 minutes.
本研究展示了涂覆刀片喷雾（CBS）质谱（MS）在对生物流体斑点中的多种目标物质进行同时分析方面的定量能力。在 CBS-MS 中，首先在 CBS 装置的薄涂层中分离并富集给定样品中的分析物。在快速冲洗刀片表面以去除残留基质后，借助溶剂快速解吸分析物，然后直接将其电喷入 MS 分析仪。仅使用 10 μL 生物流体，即可成功确定各种疼痛管理药物、受控物质和治疗药物，在 7 分钟内达到低/亚 ng·mL 级别的定量限。
