Wang Y, Mahler I, Levinson H S, Halvorson H O
Rosenstiel Basic Medical Sciences Research Center, Waltham, Massachusetts.
J Bacteriol. 1987 Oct;169(10):4848-51. doi: 10.1128/jb.169.10.4848-4851.1987.
A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to HgCl2 and to phenylmercury acetate (PMA) was expressed. Shortening the cloned fragment to 3.8 kb abolished resistance to PMA but not to HgCl2. In Bacillus subtilis, the 3.8-kb fragment produced mercuric reductase constitutively but did not produce resistance to HgCl2 or to PMA.
从一株耐汞芽孢杆菌中克隆出一个7.9千碱基（kb）的染色体片段。在大肠杆菌中，当存在携带功能性转运基因的第二个质粒时，可表达对氯化汞和乙酸苯汞（PMA）的抗性。将克隆片段缩短至3.8 kb可消除对PMA的抗性，但对氯化汞的抗性不受影响。在枯草芽孢杆菌中，3.8 kb的片段组成型产生汞还原酶，但对氯化汞或PMA均不产生抗性。
