漫浩血清群一个新血清型的基因组分析
Genomic Analysis of a New Serovar of Serogroup Manhao.
作者信息
Xu Yinghua, Zheng Huajun, Zhang Ying, Wang Yuezhu, Zhang Jinlong, Li Zhe, Cui Shenghui, Xin Xiaofang, Ye Qiang, Chang Yung-Fu, Wang Junzhi
机构信息
Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institutes for Food and Drug Control Beijing, China.
Key Laboratory of Reproduction Regulation of NPFPC, Shanghai Institute of Planned Parenthood Research, IRD, Fudan UniversityShanghai, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Zhangjiang Hi-Tech ParkShanghai, China.
出版信息
Front Microbiol. 2017 Feb 2;8:149. doi: 10.3389/fmicb.2017.00149. eCollection 2017.
Leptospirosis, caused by pathogenic spp., is recognized as an important emerging zoonotic disease throughout the world. In this study, multiple approaches were used to characterize the recently discovered serovar Heyan strain L231. This strain can infect guinea pigs and belonged to the pathogenic species . Genome sequencing analysis revealed the draft genome of 4.2 M bp with a G+C content of 40.67% for strain L231, and a total of 4,794 ORFs were identified. The strain L231 genome was found to have a larger LPS biosynthesis locus than that of strains serovar Lai and serovar Hardjobovis. Phylogenomic reconstructions showed that the evolutionary position of serovar Heyan was different from that of other serovars from serogroup Manhao. These findings may lead us to a better understanding of pathogenesis and evolution.
钩端螺旋体病由致病性钩端螺旋体属物种引起，是全球公认的一种重要的新发人畜共患病。在本研究中，采用了多种方法对最近发现的血清型合源株L231进行特征分析。该菌株可感染豚鼠，属于致病物种。基因组测序分析显示，L231菌株的基因组草图为4.2 M bp，G+C含量为40.67%，共鉴定出4794个开放阅读框。发现L231菌株的基因组比赖型菌株和哈德乔波维斯型菌株具有更大的脂多糖生物合成位点。系统发育基因组重建表明，合源血清型的进化位置与曼耗血清群的其他血清型不同。这些发现可能有助于我们更好地理解其发病机制和进化过程。
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