Nazemi Saeid, Mirzaii Mehdi, Yaslianifard Somayeh, Darban-Sarokhalil Davood, Khoramrooz Seyyed Sajjad, Norozi Pirasteh, Davardoost Fatemeh
Department of Environmental Health Engineering, School of Public Health, Shahroud University of Medical Sciences, Shahroud, Iran.
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Iran J Microbiol. 2016 Aug;8(4):219-225.
The microbiological monitoring of the water used for haemodialysis is important especially for and non-fermentative bacteria since patients with end stage renal disease (ESRD) are suffering from deteriorated function of immune system.
A total 50 water and dialysate samples were weekly collected over a period of 10 weeks from 5 sites. Total and faecal coliforms were determined by utilizing the most probable number (MPN) method. For isolation of water samples were inoculated on a BCYE medium. DNA extraction was performed and was used to amplify gene of species. Airborne bacteria were sampled using a single stage Andersen air sampler.
Out of total 50 water samples, 24 samples had bacterial contamination. The highest rate of contamination was observed in the storage tank (67 cfu/ml). was not isolated from the dialysate effluent samples. The highest rate of total bacterial count was related to the dialysate effluent and the maximum total count of coliforms was related to the reverse osmosis. The isolated bacteria were Gram-negative bacilli (mostly isolates), Gram-positive cocci (mostly ) and Gram-positive bacilli (mostly ). Six samples were contaminated with coliforms. No faecal coliform was isolated from the samples.
These results indicated that dialysis machine is an important source of contaminations such as and Therefore an efficient prevention program is needed to eliminate bacterial contamination of dialysis water system. Moreover, in haemodialysis centres, periodic surveillance programs for microbiological qualification can lead to a better planning for disinfection of haemodialysis water systems.
血液透析用水的微生物监测尤为重要，特别是对于[具体细菌名称未给出]和非发酵菌，因为终末期肾病（ESRD）患者的免疫系统功能已恶化。
在10周内每周从5个位点总共采集50份水和透析液样本。采用最可能数（MPN）法测定总大肠菌群和粪大肠菌群。为分离[具体细菌名称未给出]，将水样接种于BCYE培养基上。进行DNA提取并用于扩增[具体细菌名称未给出]物种的[具体基因未给出]基因。使用单级安德森空气采样器对空气中的细菌进行采样。
在总共50份水样中，有24份样本受到细菌污染。在储水箱中观察到[具体细菌名称未给出]污染率最高（67 cfu/ml）。未从透析液流出样本中分离出[具体细菌名称未给出]。总细菌计数的最高率与透析液流出物有关，大肠菌群的最大总数与反渗透有关。分离出的细菌为革兰氏阴性杆菌（主要是[具体细菌名称未给出]分离株）、革兰氏阳性球菌（主要是[具体细菌名称未给出]）和革兰氏阳性杆菌（主要是[具体细菌名称未给出]）。6份样本被大肠菌群污染。样本中未分离出粪大肠菌群。
这些结果表明透析机是诸如[具体细菌名称未给出]和[具体细菌名称未给出]等污染物的重要来源。因此，需要一个有效的预防计划来消除透析水系统的细菌污染。此外，在血液透析中心，微生物鉴定的定期监测计划可以更好地规划血液透析水系统的消毒工作。
