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Rapid technique of DNA-DNA in situ hybridisation on formalin fixed tissue sections using microwave irradiation.

作者信息

Coates P J, Hall P A, Butler M G, D'Ardenne A J

机构信息

Department of Histopathology, St. Bartholomew's Hospital, London.

出版信息

J Clin Pathol. 1987 Aug;40(8):865-9. doi: 10.1136/jcp.40.8.865.

DOI:10.1136/jcp.40.8.865
PMID:2821079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1141126/
Abstract

The relative sensitivities of different protocols for detecting cytomegalovirus nucleic acid sequences in histological specimens, using a biotinylated cDNA probe, were assessed. Several commonly used pre-treatment steps were not essential, nor was the use of a highly sensitive detection system. The choice of enzyme used for proteolytic digestion of tissue seems to be important, and increasing the temperature of denaturation of tissue and probe DNA to above 100 degrees C greatly increased the sensitivity of the method. Difficulties in achieving such high temperatures in a controlled manner were overcome by the use of a rapid microwave heating method that can be used routinely in laboratories. This technique detected cytomegalovirus infections in formalin fixed, paraffin processed tissue sections within a single working day.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b78/1141126/3403f56e3258/jclinpath00328-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b78/1141126/5c9275035c62/jclinpath00328-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b78/1141126/3403f56e3258/jclinpath00328-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b78/1141126/5c9275035c62/jclinpath00328-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b78/1141126/3403f56e3258/jclinpath00328-0054-a.jpg

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本文引用的文献

1
Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.通过使用与生物素化核苷酸类似物的原位杂交技术，在鸡肌肉组织培养物中观察到肌动蛋白基因的表达。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7331-5. doi: 10.1073/pnas.79.23.7331.
2
Widespread presence of histologically occult cytomegalovirus.组织学隐匿性巨细胞病毒广泛存在。
Hum Pathol. 1984 May;15(5):430-9. doi: 10.1016/s0046-8177(84)80076-3.
3
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.
Histochem J. 1988 Jun-Jul;20(6-7):341-6. doi: 10.1007/BF01002727.
4
Localization of cytomegalovirus DNA in plastic-embedded sections by in situ hybridization. A methodologic study.通过原位杂交在塑料包埋切片中定位巨细胞病毒DNA。一项方法学研究。
Am J Pathol. 1989 Feb;134(2):457-63.
5
Use of immunocytochemistry and biotinylated in situ hybridisation for detecting measles virus in central nervous system tissue.利用免疫细胞化学和生物素化原位杂交技术检测中枢神经系统组织中的麻疹病毒。
J Clin Pathol. 1990 Apr;43(4):329-33. doi: 10.1136/jcp.43.4.329.
6
DNA hybridisation of routinely processed tissue for detecting HPV DNA in anal squamous cell carcinomas over 40 years.40多年来，用于检测肛管鳞状细胞癌中HPV DNA的常规处理组织的DNA杂交技术。
J Clin Pathol. 1990 Feb;43(2):133-6. doi: 10.1136/jcp.43.2.133.
7
Improved detection of HBV DNA by PCR after microwave treatment of serum.血清经微波处理后通过聚合酶链反应（PCR）对乙肝病毒（HBV）DNA检测的改进
Nucleic Acids Res. 1991 Jul 25;19(14):4006. doi: 10.1093/nar/19.14.4006.
8
Microwave fixation of water-cooled insect tissues for immunohistochemistry.
Histochem J. 1990 Jun-Jul;22(6-7):313-20. doi: 10.1007/BF01003163.
9
Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridisation.通过非放射性原位杂交检测石蜡切片中单个拷贝的爱泼斯坦-巴尔病毒
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10
In situ hybridisation of EBV DNA-DNA hybrids using wet heat in polypropylene containers.在聚丙烯容器中使用湿热进行EBV DNA-DNA杂交体的原位杂交。
J Clin Pathol. 1992 Dec;45(12):1099-104. doi: 10.1136/jcp.45.12.1099.
使用生物素标记的杂交探针检测培养细胞和石蜡包埋组织切片中的病毒基因组。
Virology. 1983 Apr 15;126(1):32-50. doi: 10.1016/0042-6822(83)90460-9.
4
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7
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9
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Am J Surg Pathol. 1986 Jan;10(1):1-8. doi: 10.1097/00000478-198601000-00001.
10
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J Histochem Cytochem. 1986 Jan;34(1):33-8. doi: 10.1177/34.1.3001177.