四种技术显示巨细胞病毒与卡波西肉瘤之间病因学关联的不合理性。
Implausibility of an aetiological association between cytomegalovirus and Kaposi's sarcoma shown by four techniques.
作者信息
Van den Berg F, Schipper M, Jiwa M, Rook R, Van de Rijke F, Tigges B
机构信息
Department of Pathology AMC, University of Amsterdam, The Netherlands.
出版信息
J Clin Pathol. 1989 Feb;42(2):128-31. doi: 10.1136/jcp.42.2.128.
The presence of cytomegalovirus (CMV) was analysed in either lymph node or skin and lung tissue necropsy specimens affected by Kaposi's sarcoma, from 10 patients who had died of AIDS. The different detection techniques used were: (i) immunohistochemical demonstration of CMV immediate early antigen (IEA); (ii) in situ hybridisation with a biotinylated CMV DNA probe; (iii) Southern blot hybridisation of DNA extracted from sequential tissue sections; and (iv) polymerase chain reaction (PCR) with CMV specific primers on the DNA samples. The results of these analyses were compared with the postmortem data on CMV obtained by infectious particle assays and histological examination, especially of adrenal glands of the same patients. The results of the various detection methods correlated very well, yielding a combined score of six of 10 patients positive for CMV; there did not seem to be any association between the presence of CMV and the occurrence of Kaposi's sarcoma in our patients.
对10例死于艾滋病的患者进行尸检，分析其受卡波西肉瘤影响的淋巴结、皮肤和肺组织标本中是否存在巨细胞病毒（CMV）。所采用的不同检测技术如下：（i）巨细胞病毒即刻早期抗原（IEA）的免疫组化显示；（ii）用生物素化的巨细胞病毒DNA探针进行原位杂交；（iii）对连续组织切片提取的DNA进行Southern印迹杂交；（iv）用巨细胞病毒特异性引物对DNA样本进行聚合酶链反应（PCR）。将这些分析结果与通过感染性颗粒检测和组织学检查（特别是对同一患者的肾上腺）获得的关于巨细胞病毒的尸检数据进行比较。各种检测方法的结果相关性非常好，10例患者中有6例巨细胞病毒检测呈阳性；在我们的患者中，巨细胞病毒的存在与卡波西肉瘤的发生之间似乎没有任何关联。
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