McQuaid S, Isserte S, Allan G M, Taylor M J, Allen I V, Cosby S L
Queen's University of Belfast, Multiple Sclerosis Laboratory, Royal Victoria Hospital.
J Clin Pathol. 1990 Apr;43(4):329-33. doi: 10.1136/jcp.43.4.329.
Optimised immunocytochemical (ICC) and in situ hybridisation (ISH) protocols for long term, formalin fixed, central nervous system tissue infected with measles virus were developed. The effectiveness of 10 proteases for the enzymatic unmasking of formalin fixed antigen and nucleic acid was investigated. Protease VIII gave maximal signal generation with optimal tissue preservation and no background staining for both techniques. The use of a microwave oven as an additional pre-hybridisation step for RNA-RNA in situ hybridisation produced a significant increase in the number of cells labelled for genomic RNA. The ability to show the presence of antigen and nucleic acid in long term, formalin fixed tissue facilitates the use of stored necropsy material available in pathology departments for ICC and ISH investigations.
开发了针对长期用福尔马林固定的、感染麻疹病毒的中枢神经系统组织的优化免疫细胞化学（ICC）和原位杂交（ISH）方案。研究了10种蛋白酶对福尔马林固定抗原和核酸进行酶解暴露的有效性。蛋白酶VIII在两种技术中均能产生最大信号，同时能实现最佳的组织保存且无背景染色。在RNA - RNA原位杂交中，使用微波炉作为额外的预杂交步骤，可显著增加标记基因组RNA的细胞数量。能够在长期福尔马林固定组织中显示抗原和核酸的存在，这有助于利用病理科现有的尸检材料进行ICC和ISH研究。
