Upcroft P
Queensland Institute of Medical Research, Herston, Brisbane, Australia.
J Gen Virol. 1987 Sep;68 ( Pt 9):2477-80. doi: 10.1099/0022-1317-68-9-2477.
The uptake of simian virus 40 (SV40) virions to the nucleus, the site of viral replication, proceeds via engulfment at the cytoplasmic membrane and transport in monopinocytotic vesicles through the cytoplasm to the nuclear membrane. In the case of Semliki Forest virus and poliovirus which undergo primary endocytosis in a similar manner, neutralization of the acid pH in these vesicles abolishes viral infectivity. We have examined the effects of the lysosomotropic agents chloroquine and ammonium chloride on the uptake of SV40 and find that neutralization of the acid pH in cellular organelles has no effect on the progress of SV40 infection. Although the initial endocytotic pathway appears similar for the viruses, the vesicular transport of SV40 to the nucleus proceeds, therefore, via an alternative endocytotic compartment which is not inhibited by increasing the endosomal pH.
猿猴病毒40（SV40）病毒粒子摄取至病毒复制场所细胞核的过程，是通过在细胞质膜处被吞噬，并在单泡小体中经细胞质运输至核膜来进行的。对于以类似方式进行初级内吞作用的塞姆利基森林病毒和脊髓灰质炎病毒而言，这些小泡中酸性pH的中和会消除病毒感染性。我们研究了溶酶体促渗剂氯喹和氯化铵对SV40摄取的影响，发现细胞器中酸性pH的中和对SV40感染进程没有影响。因此，尽管这些病毒的初始内吞途径看似相似，但SV40向细胞核的小泡运输是通过一个替代的内吞区室进行的，该区域不会因内体pH升高而受到抑制。
