Heinz B A, Cliver D O, Donohoe B
Food Research Institute, University of Wisconsin, Madison 53706.
J Gen Virol. 1987 Sep;68 ( Pt 9):2495-9. doi: 10.1099/0022-1317-68-9-2495.
Organ explants of porcine ileum were cultured in different media for up to 48 h. Tissue preservation was evaluated by light microscopy and by transmission and scanning electron microscopy. Cellular structure was well maintained after incubation for 48 h in CMRL-1066 supplemented with insulin and cortisone. Explants of absorptive or lymphoid tissue from young or adult pigs were incubated with either coxsackievirus B5 (which is infectious for swine) or human poliovirus type 1 (which served as a control) for 24 h at 37 degrees C. Progeny virus was detected by plaque assay. Replication was most evident in the absorptive tissue explants from young pigs. In tissues from adults, replication occurred equally well in absorptive and lymphoid tissues. Infection in explants was inefficient, and the yield of progeny virus was low.
将猪回肠的器官外植体在不同培养基中培养长达48小时。通过光学显微镜以及透射和扫描电子显微镜评估组织保存情况。在补充了胰岛素和可的松的CMRL - 1066中孵育48小时后，细胞结构得到良好维持。将幼年或成年猪的吸收性或淋巴组织外植体在37℃下与柯萨奇病毒B5（对猪具有传染性）或人脊髓灰质炎病毒1型（用作对照）孵育24小时。通过蚀斑测定法检测子代病毒。复制在幼年猪的吸收性组织外植体中最为明显。在成年猪的组织中，吸收性和淋巴组织中的复制情况同样良好。外植体中的感染效率低下，子代病毒产量较低。
