在哺乳动物细胞中从克隆的互补脱氧核糖核酸生产传染性猪水疱病病毒。
Production of infectious swine vesicular disease virus from cloned cDNA in mammalian cells.
作者信息
Inoue T, Yamaguchi S, Saeki T, Sekiguchi K
机构信息
Exotic Disease Research Division, National Institute of Animal Health, Tokyo, Japan.
出版信息
J Gen Virol. 1990 Aug;71 ( Pt 8):1835-8. doi: 10.1099/0022-1317-71-8-1835.
PMID:2167939
Abstract
Full-length cDNA clones of the swine vesicular disease virus (SVDV) were constructed from subgenomic cDNA clones in the expression vector pSVL (pSVLS00). The direct transfection of mammalian cells with plasmid pSVLS00 results in the production of infectious virus. The recovered virus was neutralized completely by anti-SVDV guinea-pig serum, but did show a difference in plaque morphology from the parental virus.
摘要
猪水泡病病毒（SVDV）的全长cDNA克隆是从表达载体pSVL（pSVLS00）中的亚基因组cDNA克隆构建而成。用质粒pSVLS00直接转染哺乳动物细胞可产生感染性病毒。回收的病毒能被抗SVDV豚鼠血清完全中和，但在空斑形态上与亲代病毒确实存在差异。
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