Yoshii T, Natori K, Kono R
J Gen Virol. 1977 Sep;36(3):377-84. doi: 10.1099/0022-1317-36-3-377.
Replication of the strain J 670/71 of enterovirus 70 (EV70) in non-primate cell cultures at 33 degrees C was studied using strain L (mouse), BHK21 (hamster), RK13 (rabbit), RK17 (rabbit), PK15 (porcine), IB-RS-2 (porcine), ESK (porcine), MDBK (bovine), and BK1 (primary bovine) together with that of the LSc, 2ab strain of poliovrus type 1 (PV1) as a control. All the cells tested adsorbed from 54 to 90% of EV70. The replication with complete c.p.e. was evident in RK13, RK17 and BK1 cells; replication without c.p.e. was shown in L, BHK21, IB-RS-2 and ESK; but PK15 and MDBK were non-permissive despite a high virus adsorption rate. On the contrary, none of these non-primate cells allowed the adsorption and growth of PV1. One-step growth of EV70 in RK13 was almost identical with that in the primate cells. Two other strains of EV70 were found having similar host range in vitro. Therefore, it is concluded that EV70 has a wider host range in vitro than ordinary human enteroviruses, and its implication is discussed.
利用L株（小鼠）、BHK21（仓鼠）、RK13（兔）、RK17（兔）、PK15（猪）、IB-RS-2（猪）、ESK（猪）、MDBK（牛）和BK1（原代牛）细胞培养物，以及作为对照的脊髓灰质炎病毒1型（PV1）的LSc、2ab株，研究了肠道病毒70型（EV70）J 670/71株在33℃下在非灵长类细胞培养物中的复制情况。所有测试细胞对EV70的吸附率为54%至90%。在RK13、RK17和BK1细胞中可见伴有完全细胞病变效应（c.p.e.）的复制；在L、BHK21、IB-RS-2和ESK细胞中显示有不伴有c.p.e.的复制；但尽管病毒吸附率高，PK15和MDBK细胞却不支持病毒复制。相反，这些非灵长类细胞均不允许PV1吸附和生长。EV70在RK13细胞中的一步生长情况与在灵长类细胞中的几乎相同。另外还发现两株EV70在体外具有相似的宿主范围。因此，得出结论，EV70在体外的宿主范围比普通人类肠道病毒更广，并对其意义进行了讨论。
