N6, O2'-二丁酰环磷腺苷酸（DBcAMP）的致突变性研究。

Mutagenicity studies with N6, O2'-dibutyryl cyclic adenosine 3',5'-monophosphate (DBcAMP).

作者信息

Shimada H, Ebine Y

机构信息

Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.

出版信息

Mutat Res. 1987 Sep;192(1):1-6. doi: 10.1016/0165-7992(87)90117-5.

DOI:10.1016/0165-7992(87)90117-5

PMID:2821395

Abstract

N6,O2'-Dibutyryl cyclic adenosine 3,5-monophosphate (DBcAMP) was studied for mutagenicity using the rec assay, the Ames method, and in vitro cytogenetics. DBcAMP had no mutagenic effect on B. subtilis in the rec assay, or on S. typhimurium (TA1535, TA1537, TA1538, TA98 and TA100) or E. coli (WP2 uvrA). In the cytogenetic study, a significant increase in chromosomal aberrations was observed at a concentration of 50,000 micrograms/ml, but it was considered that this effect could be attributed to the secondary effect of the high osmotic pressure in the culture medium. These results suggest that DBcAMP has no mutagenic potential.

摘要

使用rec测定法、艾姆斯试验法和体外细胞遗传学方法研究了N6,O2'-二丁酰环磷腺苷（DBcAMP）的致突变性。在rec测定法中，DBcAMP对枯草芽孢杆菌没有致突变作用，对鼠伤寒沙门氏菌（TA1535、TA1537、TA1538、TA98和TA100）或大肠杆菌（WP2 uvrA）也没有致突变作用。在细胞遗传学研究中，在浓度为50000微克/毫升时观察到染色体畸变显著增加，但认为这种作用可能归因于培养基中高渗透压的次生效应。这些结果表明DBcAMP没有致突变潜力。