Landauer M R, Davis H D, Dominitz J A, Weiss J F
Department of Behavioral Sciences, Armed Forces Radiobiology Research Institute, Bethesda, MD 20814-5145.
Pharmacol Biochem Behav. 1987 Jul;27(3):573-6. doi: 10.1016/0091-3057(87)90370-4.
The effects of the radioprotector S-2-(3-aminopropylamino)ethylphosphorothioic acid (WR-2721) on locomotor activity were evaluated in CD2F1 male mice. Separate groups of animals (N = 10/group) received an IP injection of vehicle, 25, 50, 100, 200, or 400 mg/kg of WR-2721 immediately before testing. Horizontal and vertical activity were measured using a Digiscan automated animal activity monitor. The latency to onset and duration of action of each dose of the radioprotector were recorded. For both behavioral measures, a significant reduction was observed in activity at doses of 200 and 400 mg/kg. A dose of 200 mg/kg had a 12- to 14-min latency to onset and significantly reduced behavioral activity for 3 hr. Mice injected with 400 mg/kg exhibited locomotor deficits within 8-10 min and were affected for up to 9 hr. The ED50 for horizontal and vertical activities at 1 hr postinjection were determined to be 271 and 105 mg/kg, respectively. The results demonstrate that significant reductions in locomotor activity are exhibited at doses of 200 mg/kg or more and that vertical activity was more sensitive to the disruptive effects of WR-2721 than was horizontal activity.
在CD2F1雄性小鼠中评估了辐射防护剂S-2-(3-氨丙基氨基)乙硫代磷酸（WR-2721）对运动活动的影响。将动物分成不同组（每组N = 10），在测试前立即腹腔注射赋形剂、25、50、100、200或400 mg/kg的WR-2721。使用Digiscan自动动物活动监测仪测量水平和垂直活动。记录每种剂量辐射防护剂的起效潜伏期和作用持续时间。对于这两种行为指标，在200和400 mg/kg剂量下观察到活动显著减少。200 mg/kg的剂量起效潜伏期为12至14分钟，行为活动显著减少达3小时。注射400 mg/kg的小鼠在8至10分钟内出现运动缺陷，影响长达9小时。注射后1小时水平和垂直活动的半数有效剂量（ED50）分别确定为271和105 mg/kg。结果表明，200 mg/kg或更高剂量时运动活动显著减少，并且垂直活动比水平活动对WR-2721的破坏作用更敏感。
