Sadri Donia, Farhadi Sareh, Shahabi Zahra, Sarshar Samaneh
Department of Oral and Maxillofacial Pathology, Dental Branch of Tehran, Islamic Azad University, Tehran, Iran.
Open Dent J. 2016 Dec 30;10:752-759. doi: 10.2174/1874210601610010752. eCollection 2016.
The recent scientific reports have shown that angiogenesis can affect biological behavior of pathologic lesions.
Regarding unique clinical outcome of Odontogenic keratocyst (OKC), the present study was aimed to compare angiogenesis in Odontogenic keratocyst and Dentigerous cyst (DC).
In this experimental study, tissue sections of 46 samples of OKC and DC were stained through immunohistochemical method using Vascular Endothelial Growth Factor (VEGF) antibody. VEGF expression was evaluated in epithelial cells, fibroblasts and endothelial cells. The average percentage of stained cells in any samples was categorized to 3 groups as follows: SCORE 0: 10% of cells or less are positive. SCORE 1: 10 to 50% of cells are positive. SCORE 2: more than 50% of cells are positive. Mann-U-Whitney, T-test and chi-square was used for statistical analysis.
The average of VEGF expression in 24 samples of DC was 20.2% and in 22 samples of OKC was 52.6%, respectively. The average of VEGF expression in these two cysts had statistical significant differences. (PV= 0.045). There was significant statistical differences between two cysts in the terms of VEGF SCORE (PV= 0.000). OKC samples had significantly higher SCORE for the purpose of VEGF incidence than DC. Also, there were no differences between VEGF expression in epithelial cells of two cysts (PV= 0.268) there were significant statistical differences between two cysts in terms of endothelial cell staining. The endothelial cell staining was significantly higher in OKC than DC (PV= 0.037%).
Regarding higher expression of Vascular Endothelial Growth factor in OKC than DC, it seems that angiogenesis may have great impression on clinical outcome of OKC.
近期科学报告显示血管生成可影响病理病变的生物学行为。
鉴于牙源性角化囊肿（OKC）独特的临床结局，本研究旨在比较牙源性角化囊肿和含牙囊肿（DC）中的血管生成情况。
在本实验研究中，采用血管内皮生长因子（VEGF）抗体，通过免疫组织化学方法对46例OKC和DC样本的组织切片进行染色。评估VEGF在上皮细胞、成纤维细胞和内皮细胞中的表达。将任何样本中染色细胞的平均百分比分为以下3组：评分0：10%或更少的细胞为阳性。评分1：10%至50%的细胞为阳性。评分2：超过50%的细胞为阳性。采用曼-惠特尼检验、t检验和卡方检验进行统计分析。
24例DC样本中VEGF表达的平均值为20.2%，22例OKC样本中VEGF表达的平均值为52.6%。这两种囊肿中VEGF表达的平均值存在统计学显著差异（P值=0.045）。在VEGF评分方面，两种囊肿之间存在显著统计学差异（P值=0.000）。就VEGF发生率而言，OKC样本的评分显著高于DC。此外，两种囊肿上皮细胞中的VEGF表达无差异（P值=0.268），在血管内皮细胞染色方面，两种囊肿之间存在显著统计学差异。OKC中的血管内皮细胞染色显著高于DC（P值=0.037%）。
鉴于OKC中血管内皮生长因子的表达高于DC，血管生成似乎可能对OKC的临床结局产生重大影响。
