1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland. [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland. [3] National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland.
cole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland.
Nat Chem Biol. 2014 Jul;10(7):598-603. doi: 10.1038/nchembio.1554. Epub 2014 Jun 8.
For many drugs, finding the balance between efficacy and toxicity requires monitoring their concentrations in the patient's blood. Quantifying drug levels at the bedside or at home would have advantages in terms of therapeutic outcome and convenience, but current techniques require the setting of a diagnostic laboratory. We have developed semisynthetic bioluminescent sensors that permit precise measurements of drug concentrations in patient samples by spotting minimal volumes on paper and recording the signal using a simple point-and-shoot camera. Our sensors have a modular design consisting of a protein-based and a synthetic part and can be engineered to selectively recognize a wide range of drugs, including immunosuppressants, antiepileptics, anticancer agents and antiarrhythmics. This low-cost point-of-care method could make therapies safer, increase the convenience of doctors and patients and make therapeutic drug monitoring available in regions with poor infrastructure.
对于许多药物来说，在疗效和毒性之间找到平衡需要监测患者血液中的药物浓度。在床边或家中定量检测药物水平在治疗效果和便利性方面具有优势，但目前的技术需要在诊断实验室中进行。我们已经开发出半合成生物发光传感器，通过在纸上点取最小体积的样本并使用简单的点按拍摄相机记录信号，可以精确测量患者样本中的药物浓度。我们的传感器具有模块化设计，由基于蛋白质的部分和合成部分组成，可以设计成选择性识别包括免疫抑制剂、抗癫痫药、抗癌药和抗心律失常药在内的广泛药物。这种低成本的即时护理方法可以使治疗更安全，增加医生和患者的便利性，并使治疗药物监测在基础设施较差的地区得以实现。
