卡托普利明显增加而顺铂明显降低人工尿液溶液中的人白蛋白浓度。
Captopril apparently increase and cisplatin apparently decrease human albumin concentration in artificial urinary solutions.
作者信息
Kuzioła Rafał, Marczewska Barbara, Marczewski Krzysztof
机构信息
Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, Lublin, Poland.
Pope John Paul II Regional Hospital in Zamość and Faculty of Medical Science Lublin University of Economy and Innovations, Lublin, Poland.
出版信息
J Clin Lab Anal. 2017 Nov;31(6). doi: 10.1002/jcla.22127. Epub 2017 Feb 20.
BACKGROUND
Measurement of urinary albumin (HSA) is very important in diagnostic of kidney diseases. Much less is known about the possible impact of substances present in urine together with albumin on the results of measurements.
METHODS
We investigated the effect of the presence of captopril and cisplatin in the solution on the result of the determination of HSA by native polyacrylamide gel electrophoresis. Protein conformation in the absence and presence of the drugs was examined using Fourier Transform Infrared Spectroscopy (FTIR).
RESULTS
The presence of captopril apparently increases HSA concentration while cisplatin causes an apparent decrease in the HSA concentration. The presence of both drugs also influence the secondary structure forms of HSA albumin investigated by FTIR.
CONCLUSION
Both drugs tested in the concentration of human use can have an impact on the results of determination of albumin in urine which can influence clinical decision.
背景
尿白蛋白（HSA）的测量在肾脏疾病诊断中非常重要。对于尿液中与白蛋白同时存在的物质对测量结果可能产生的影响，人们了解得要少得多。
方法
我们研究了溶液中卡托普利和顺铂的存在对通过天然聚丙烯酰胺凝胶电泳测定HSA结果的影响。使用傅里叶变换红外光谱（FTIR）检测了在有无药物情况下蛋白质的构象。
结果
卡托普利的存在明显增加了HSA浓度，而顺铂导致HSA浓度明显降低。两种药物的存在也影响了通过FTIR研究的HSA白蛋白的二级结构形式。
结论
在人体使用浓度下测试的这两种药物都可能对尿液中白蛋白的测定结果产生影响，进而可能影响临床决策。
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