Mulumba-Mfumu Leopold K, Achenbach Jenna E, Mauldin Matthew R, Dixon Linda K, Tshilenge Curé Georges, Thiry Etienne, Moreno Noelia, Blanco Esther, Saegerman Claude, Lamien Charles E, Diallo Adama
Central Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of Congo.
Research Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium.
Viruses. 2017 Feb 18;9(2):31. doi: 10.3390/v9020031.
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated-except for Sardinia-followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the gene (p72 protein), central hypervariable region (CVR) of the gene, and the gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants.
非洲猪瘟（ASF）是家猪的一种毁灭性疾病。它是一种具有社会经济重要性的疾病，最初在肯尼亚被描述，但随后在撒哈拉以南的大多数国家都有报道。ASF通过多次传入传播到欧洲、南美洲和加勒比地区，最初除了撒丁岛外都被根除了，随后在2007年又重新传入欧洲。在这项对刚果民主共和国境内ASF的研究中，对2005年至2012年间采集的62份家猪样本进行了病毒DNA检测，并在多个位点进行了测序：基因的C端（p72蛋白）、基因的中央高变区（CVR）和基因（p54蛋白）。系统发育分析确定了三种流行基因型：I型（占样本的64.5%）、IX型（32.3%）和XIV型（3.2%）。这是该国存在IX型和XIV型基因型的首个证据。对CVR的检测揭示了基因型内的高度变异，共鉴定出19种变体。
