Kumano Y, Yamamoto M, Mori R
Department of Virology, School of Medicine, Kyushu University, Fukuoka, Japan.
Antiviral Res. 1987 Jun;7(5):289-301. doi: 10.1016/0166-3542(87)90012-x.
A recombinant murine interferon -beta (rMuIFN-beta) was used to suppress the development of skin lesions and death of mice after challenge with herpes simplex virus (HSV) type 1 (HSV-1). Depilated female BALB/c mice were inoculated intradermally with HSV-1, Hayashida strain, and were administered various concentrations of interferon (IFN) intraperitoneally 3 h later. The treatment with IFN was given once a day for 10 successive days. Under the conditions in which almost all control mice died after development of severe zosteriform skin lesions, the mortality of mice treated with IFN (8 X 10(5) or 8 X 10(4) U/mouse) was less than 50% (9/20 and 4/10, respectively), though all mice treated with a lower dose of IFN (8 X 10(3) U/mouse) died. Titration revealed that there was no significant suppression of virus growth by IFN in the skin or dorsal root ganglia, but it was significantly suppressed in the brain. The protective effect of IFN was enhanced when it was used in combination with human anti-HSV antibody having a neutralizing titer (NT) of 1:16. All mice treated with IFN (8 X 10(5) U/mouse) and antibody (NT, 1:16) survived, and only 40% of them developed slight zosteriform skin lesions. The effect of the combination was observed even when both IFN and antibody were diluted 1:10. The protective effect of IFN was also observed when athymic nude mice were used as the host. In this system, though the IFN-treated nude mice survived significantly longer than the controls, they finally died. In antibody- or acyclovir (ACV)-treated nude mice, there was also a prolongation of survival time as compared with control mice. The effect of antibody was enhanced by the addition of IFN, but IFN did not potentiate the effect of ACV.
一种重组鼠干扰素-β（rMuIFN-β）被用于抑制1型单纯疱疹病毒（HSV-1）攻击后小鼠皮肤损伤的发展和死亡。将脱毛的雌性BALB/c小鼠皮内接种HSV-1 Hayashida株，3小时后腹腔注射不同浓度的干扰素（IFN）。IFN治疗连续10天每天给药一次。在几乎所有对照小鼠在出现严重带状疱疹样皮肤损伤后死亡的条件下，用IFN（8×(10^5)或8×(10^4) U/小鼠）治疗的小鼠死亡率低于50%（分别为9/20和4/10），而用较低剂量IFN（8×(10^3) U/小鼠）治疗的所有小鼠均死亡。滴定显示，IFN对皮肤或背根神经节中的病毒生长没有显著抑制作用，但在脑中病毒生长被显著抑制。当IFN与中和效价（NT）为1:16的人抗HSV抗体联合使用时，IFN的保护作用增强。所有用IFN（8×(10^5) U/小鼠）和抗体（NT，1:16）治疗的小鼠均存活，其中只有40%出现轻微的带状疱疹样皮肤损伤。即使IFN和抗体都稀释1:10，也观察到了联合用药的效果。当无胸腺裸鼠作为宿主时，也观察到了IFN的保护作用。在这个系统中，虽然用IFN治疗的裸鼠存活时间明显长于对照小鼠，但它们最终还是死亡了。与对照小鼠相比，在抗体或阿昔洛韦（ACV）治疗的裸鼠中，存活时间也有延长。添加IFN可增强抗体的作用，但IFN不能增强ACV的作用。
