Yamamoto M, Tang J L, Kumano Y, Mori R, Kino Y
Antiviral Res. 1986 Jul;6(4):223-31. doi: 10.1016/0166-3542(86)90003-3.
The effect of monoclonal antibody (MCA) to glycoprotein gB of herpes simplex virus (HSV) was studied in athymic nude mice inoculated with HSV intracutaneously in the midflank. HS1, the MCA used in the study, had a high neutralizing titer (1:2048) and had antibody-dependent cell-mediated cytotoxicity. HS1 was injected intraperitoneally at various intervals after HSV infection. HS1 injected 3 h after infection inhibited the development of skin lesions and most mice survived. Administration of HS1 at the time the local skin erosions appeared at the inoculated site (4-7 days after infection) was also effective, and in four of eight mice skin lesions completely healed. Furthermore, in three of four mice that survived, latent infections in the ganglia were also prevented as evidenced by the failure to detect HSV by co-cultivation with Vero cells. Administration of HS1 after the development of zosteriform skin lesions (5-9 days after infection) reduced virus in the ganglia and prolonged the survival time, though the disease was not completely arrested and all the mice died eventually.
在将单纯疱疹病毒（HSV）经皮接种于胁腹中部的无胸腺裸鼠中，研究了针对单纯疱疹病毒糖蛋白gB的单克隆抗体（MCA）的作用。该研究中使用的MCA即HS1，具有高中和效价（1:2048），并具有抗体依赖性细胞介导的细胞毒性。在HSV感染后的不同时间间隔腹腔注射HS1。感染后3小时注射HS1可抑制皮肤损伤的发展，大多数小鼠存活。在接种部位出现局部皮肤糜烂时（感染后4 - 7天）给予HS1也有效，8只小鼠中有4只的皮肤损伤完全愈合。此外，在存活的4只小鼠中有3只，神经节中的潜伏感染也得到了预防，这通过与Vero细胞共培养未能检测到HSV得以证明。在带状疱疹样皮肤损伤出现后（感染后5 - 9天）给予HS1可减少神经节中的病毒并延长存活时间，尽管疾病未完全停止，所有小鼠最终均死亡。
