Fujinaga S, Sugano T, Matsumoto Y, Masuho Y, Mori R
J Infect Dis. 1987 Jan;155(1):45-53. doi: 10.1093/infdis/155.1.45.
Hybridomas producing human monoclonal antibodies (MAbs) against herpes simplex virus (HSV) were established by fusing human tonsillar lymphocytes with mouse myeloma cells. Three hybridomas have been stably producing MAbs for more than 16 months. All three MAbs--H1, H2, and H3--were of the IgG1 isotype and recognized the gB glycoprotein of HSV types 1 and 2 (HSV-1 and HSV-2). MAbs H2 and H3 not only bound to the surface membrane of HSV-infected cells but also neutralized both HSV-1 and HSV-2, whereas MAb H1 had neither activity. In mouse infection experiments, MAbs H2 and H3 showed a potent protective effect against HSV-1 infection, whereas MAB H1 was less protective. Furthermore, the development of zosteriform skin lesions in athymic nude mice was suppressed by administering MAb H2. These results suggest that human MAbs might provide passive immunization against HSV infections in humans.
通过将人扁桃体淋巴细胞与小鼠骨髓瘤细胞融合，建立了产生抗单纯疱疹病毒（HSV）人单克隆抗体（MAb）的杂交瘤。三种杂交瘤已稳定产生单克隆抗体超过16个月。所有三种单克隆抗体——H1、H2和H3——均为IgG1同种型，可识别1型和2型单纯疱疹病毒（HSV - 1和HSV - 2）的gB糖蛋白。单克隆抗体H2和H3不仅与HSV感染细胞的表面膜结合，还能中和HSV - 1和HSV - 2，而单克隆抗体H1则没有任何活性。在小鼠感染实验中，单克隆抗体H2和H3对HSV - 1感染显示出强大的保护作用，而单克隆抗体H1的保护作用较弱。此外，通过给予单克隆抗体H2，可抑制无胸腺裸鼠带状疱疹样皮肤损伤的发展。这些结果表明，人单克隆抗体可能为人类提供针对HSV感染的被动免疫。
