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相似文献

1
In vivo formation of gene fusions in Pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of Mu d1 and Mu d2 fusions.恶臭假单胞菌中基因融合的体内形成以及用于直接亚克隆Mu d1和Mu d2融合体的通用广宿主载体的构建。
Appl Environ Microbiol. 1987 Jul;53(7):1649-54. doi: 10.1128/aem.53.7.1649-1654.1987.
2
Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors.大肠杆菌杂种trp-lac（tac）启动子在恶臭假单胞菌中的活性。广宿主范围、可控表达载体的构建。
Gene. 1983 Dec;26(2-3):273-82. doi: 10.1016/0378-1119(83)90197-x.
3
Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.专用质粒克隆载体。II. 广泛宿主范围、高拷贝数、源自RSF1010的载体以及用于假单胞菌基因克隆的宿主-载体系统。
Gene. 1981 Dec;16(1-3):237-47. doi: 10.1016/0378-1119(81)90080-9.
4
Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli.食油假单胞菌烷烃利用系统在恶臭假单胞菌和大肠杆菌中的可控功能性表达。
J Biol Chem. 1987 Dec 25;262(36):17712-8.
5
Cloning, DNA sequence analysis, and expression in Escherichia coli of the gene for mandelate racemase from Pseudomonas putida.恶臭假单胞菌扁桃酸消旋酶基因的克隆、DNA序列分析及其在大肠杆菌中的表达
Biochemistry. 1988 Jan 26;27(2):540-5. doi: 10.1021/bi00402a006.
6
Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas.克隆载体，源自一种天然存在的野油菜黄单胞菌质粒，专门为在假单胞菌中进行基因操作而设计。
Gene. 1990 Mar 1;87(1):145-9. doi: 10.1016/0378-1119(90)90507-n.
7
[Cloning and localization of the replication and mobilization regions in the D-plasmid of Pseudomonas putida pBS286 (IncP-9) with a broad host range].[恶臭假单胞菌pBS286（IncP-9）具有广泛宿主范围的D质粒中复制和转移区域的克隆与定位]
Genetika. 1988 Jun;24(6):980-92.
8
Delivery system for creation of one-step in vivo lac gene fusions in Pseudomonas spp. involved in biological control.用于在参与生物防治的假单胞菌属中一步法体内创建lac基因融合体的递送系统。
Appl Environ Microbiol. 1988 Nov;54(11):2877-80. doi: 10.1128/aem.54.11.2877-2880.1988.
9
A simple procedure for transferring genes cloned in Escherichia coli vectors into other gram-negative bacteria: phenotypic analysis and mapping of TOL plasmid gene xylK.一种将克隆于大肠杆菌载体中的基因转移至其他革兰氏阴性菌的简单方法：TOL质粒基因xylK的表型分析与定位
Gene. 1989 May 15;78(1):19-27. doi: 10.1016/0378-1119(89)90310-7.
10
Genetic analysis of chromosomal operons involved in degradation of aromatic hydrocarbons in Pseudomonas putida TMB.恶臭假单胞菌TMB中参与芳烃降解的染色体操纵子的遗传分析。
J Bacteriol. 1990 Nov;172(11):6355-62. doi: 10.1128/jb.172.11.6355-6362.1990.

引用本文的文献

1
Cyclic AMP-Independent Control of Twitching Motility in Pseudomonas aeruginosa.铜绿假单胞菌中抽动运动的环磷酸腺苷非依赖性调控
J Bacteriol. 2017 Jul 25;199(16). doi: 10.1128/JB.00188-17. Print 2017 Aug 15.
2
Type IV Pilus Alignment Subcomplex Proteins PilN and PilO Form Homo- and Heterodimers in Vivo.IV型菌毛排列亚复合体蛋白PilN和PilO在体内形成同二聚体和异二聚体。
J Biol Chem. 2016 Sep 16;291(38):19923-38. doi: 10.1074/jbc.M116.738377. Epub 2016 Jul 29.
3
Delivery system for creation of one-step in vivo lac gene fusions in Pseudomonas spp. involved in biological control.用于在参与生物防治的假单胞菌属中一步法体内创建lac基因融合体的递送系统。
Appl Environ Microbiol. 1988 Nov;54(11):2877-80. doi: 10.1128/aem.54.11.2877-2880.1988.
4
Marking the rhizopseudomonas strain 7NSK2 with a Mu d(lac) element for ecological studies.用Mu d(lac)元件标记根际假单胞菌菌株7NSK2用于生态学研究。
Appl Environ Microbiol. 1990 Apr;56(4):1046-52. doi: 10.1128/aem.56.4.1046-1052.1990.
5
Bacteriophage Mu as a genetic tool to study Erwinia amylovora pathogenicity and hypersensitive reaction on tobacco.噬菌体Mu作为研究梨火疫病菌致病性及对烟草过敏反应的遗传工具。
J Bacteriol. 1990 Feb;172(2):932-41. doi: 10.1128/jb.172.2.932-941.1990.
6
Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens.荧光假单胞菌属参与抑制植物根部病原体的特性。
Microbiol Rev. 1992 Dec;56(4):662-76. doi: 10.1128/mr.56.4.662-676.1992.

本文引用的文献

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Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.大肠杆菌K12中细菌重组过程中λ原噬菌体溶源性的分离
Genetics. 1954 Jul;39(4):429-39. doi: 10.1093/genetics/39.4.429.
2
[Processes of conjugation and recombination in Escherichia coli. I. Induction by conjugation or zygotic induction].[大肠杆菌中的接合与重组过程。I. 接合诱导或合子诱导]
Ann Inst Pasteur (Paris). 1956 Oct;91(4):486-510.
3
Plasmid screening at high colony density.高菌落密度下的质粒筛选
Gene. 1980 Jun;10(1):63-7. doi: 10.1016/0378-1119(80)90144-4.
4
Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions.利用基因融合技术对肺炎克雷伯菌固氮（nif）基因簇调控的分析。
Nature. 1980 Jul 10;286(5769):128-32. doi: 10.1038/286128a0.
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Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri.细菌生物发光：费氏弧菌功能的分离与遗传分析
Cell. 1983 Mar;32(3):773-81. doi: 10.1016/0092-8674(83)90063-6.
6
Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).利用转导噬菌体Mu d（Apr lac）在鼠伤寒沙门氏菌LT2中分离ara-lac基因融合体。
J Bacteriol. 1980 Sep;143(3):1325-31. doi: 10.1128/jb.143.3.1325-1331.1980.
7
Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.通过RP4::Mu将噬菌体Mu导入各种属的细菌以及属间基因转移。
J Bacteriol. 1981 Jan;145(1):358-68. doi: 10.1128/jb.145.1.358-368.1981.
8
Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.用大肠杆菌噬菌体Mu d1（Apr lac）感染鼠伤寒沙门氏菌：构建pyr::lac基因融合体。
J Bacteriol. 1981 Jan;145(1):299-305. doi: 10.1128/jb.145.1.299-305.1981.
9
Mutagenesis and mutation transfer induced by ultraviolet light in plasmid-cloned DNA.紫外线在质粒克隆DNA中诱导的诱变和突变转移。
Gene. 1984 Feb;27(2):213-22. doi: 10.1016/0378-1119(84)90142-2.
10
Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors.大肠杆菌杂种trp-lac（tac）启动子在恶臭假单胞菌中的活性。广宿主范围、可控表达载体的构建。
Gene. 1983 Dec;26(2-3):273-82. doi: 10.1016/0378-1119(83)90197-x.

恶臭假单胞菌中基因融合的体内形成以及用于直接亚克隆Mu d1和Mu d2融合体的通用广宿主载体的构建。

In vivo formation of gene fusions in Pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of Mu d1 and Mu d2 fusions.

作者信息

Simon V, Schumann W

机构信息

Lehrstuhl für Genetik, Universität Bayreuth, Federal Republic of Germany.

出版信息

Appl Environ Microbiol. 1987 Jul;53(7):1649-54. doi: 10.1128/aem.53.7.1649-1654.1987.

DOI:10.1128/aem.53.7.1649-1654.1987
PMID:2821901
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC203925/
Abstract

The Mu d1 and Mu d2 prophages were integrated into the conjugative broad-host-range plasmid R751. The two plasmids were then transferred into Pseudomonas putida, and derivatives carrying intact Mu prophages were recovered. After induction of Mu at 42 degrees C, both operon and gene fusions were observed on 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) plates. Broad-host-range vectors were constructed which allow direct cloning of both operon or gene fusions and their analysis in Escherichia coli and P. putida. By using one of these vectors, two operon fusions were isolated from the P. putida chromosome and comparatively analyzed in E. coli and P. putida.

摘要

Mu d1和Mu d2原噬菌体被整合到接合型广宿主范围质粒R751中。然后将这两种质粒转移到恶臭假单胞菌中，并回收携带完整Mu原噬菌体的衍生物。在42℃诱导Mu后，在5-溴-4-氯-3-吲哚基-β-D-吡喃半乳糖苷（X-Gal）平板上观察到操纵子融合和基因融合。构建了广宿主范围载体，可直接克隆操纵子或基因融合体，并在大肠杆菌和恶臭假单胞菌中进行分析。通过使用其中一种载体，从恶臭假单胞菌染色体中分离出两个操纵子融合体，并在大肠杆菌和恶臭假单胞菌中进行比较分析。