O'Sullivan D J, O'Gara F
Microbiology Department, University College, Cork, Ireland.
Appl Environ Microbiol. 1988 Nov;54(11):2877-80. doi: 10.1128/aem.54.11.2877-2880.1988.
The suicide plasmid pVA838 carrying the operon fusion transposon Tn5-lac was used as a delivery system to introduce Tn5-lac into Pseudomonas sp. strain M114. Random, in vivo lac gene fusions were successfully isolated in a one-step conjugation approach with this vector system. Tn5-lac-containing exconjugants were recovered at a frequency of approximately 10(-7) per recipient. However, when the mating temperature was increased from the normal growth temperature (28 degrees C) to 34 degrees C, the frequency was increased to approximately 10(-4) per recipient. A number of in vivo lac gene fusions were isolated and characterized in strain M114, a potentially important bacterium for biological control purposes.
携带操纵子融合转座子Tn5-lac的自杀质粒pVA838被用作一种递送系统，将Tn5-lac导入假单胞菌属菌株M114。通过该载体系统的一步接合方法成功分离出随机的体内lac基因融合体。含Tn5-lac的接合后体以每受体约10(-7)的频率回收。然而，当交配温度从正常生长温度（28℃）提高到34℃时，频率增加到每受体约10(-4)。在菌株M114（一种对生物防治具有潜在重要性的细菌）中分离并鉴定了许多体内lac基因融合体。
