利用基因融合技术对肺炎克雷伯菌固氮（nif）基因簇调控的分析。
Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions.
作者信息
Dixon R, Eady R R, Espin G, Hill S, Iaccarino M, Kahn D, Merrick M
出版信息
Nature. 1980 Jul 10;286(5769):128-32. doi: 10.1038/286128a0.
DOI:10.1038/286128a0
PMID:6995849
Abstract
Gene fusions in which the lac genes are under the control of each promoter in the Klebsiella pneumoniae, nitrogen fixation (nif) gene cluster have been constructed. These fusions have been used to examine positive control of the cluster and the response of individual genes to repression by ammonia and oxygen. De-repression of nif transcriptional units is coordinate and molybdate is required for maximal expression of the structural gene operon, which is autogenously regulated.
摘要
已构建了基因融合体，其中乳糖操纵子基因受肺炎克雷伯菌固氮（nif）基因簇中每个启动子的控制。这些融合体已用于研究该基因簇的正调控以及各个基因对氨和氧抑制的反应。nif转录单位的去阻遏是协同的，并且钼酸盐是结构基因操纵子最大表达所必需的，该操纵子是自我调节的。
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