Srimachai Sirintorn, Devaux Sylvie, Demougeot Celine, Kumphune Sarawut, Ullrich Nina D, Niggli Ernst, Ingkaninan Kornkanok, Kamkaew Natakorn, Scholfield C Norman, Tapechum Sompol, Chootip Krongkarn
Department of Physiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
BMC Complement Altern Med. 2017 Feb 20;17(1):117. doi: 10.1186/s12906-017-1637-z.
This study explored Bacopa monnieri, a medicinal Ayurvedic herb, as a cardioprotectant against ischemia/reperfusion injury using cardiac function and coronary flow as end-points.
In normal isolated rat hearts, coronary flow, left ventricular developed pressure, heart rate, and functional recovery were measured using the Langendorff preparation. Hearts were perfused with either (i) Krebs-Henseleit (normal) solution, (control), or with 30, 100 μg/ml B. monnieri ethanolic extract (30 min), or (ii) with normal solution or extract for 10 min preceding no-perfusion ischemia (30 min) followed by reperfusion (30 min) with normal solution. Infarct volumes were measured by triphenyltetrazolium staining. L-type Ca-currents (I) were measured by whole-cell patching in HL-1 cells, a mouse atrial cardiomyocyte cell line. Cytotoxicity of B. monnieri was assessed in rat isolated ventricular myocytes by trypan blue exclusion.
In normally perfused hearts, B. monnieri increased coronary flow by 63 ± 13% (30 μg/ml) and 216 ± 21% (100 μg/ml), compared to control (5 ± 3%) (n = 8-10, p < 0.001). B. monnieri treatment preceding ischemia/reperfusion improved left ventricular developed pressure by 84 ± 10% (30 μg/ml), 82 ± 10% (100 μg/ml) and 52 ± 6% (control) compared to pre- ischemia/reperfusion. Similarly, functional recovery showed a sustained increase. Moreover, B. monnieri (100 μg/ml) reduced the percentage of infarct size from 51 ± 2% (control) to 25 ± 2% (n = 6-8, p < 0.0001). B. monnieri (100 μg/ml) reduced I by 63 ± 4% in HL-1 cells. Ventricular myocyte survival decreased at higher concentrations (50-1000 μg/ml) B. monnieri.
B. monnieri improves myocardial function following ischemia/reperfusion injury through recovery of coronary blood flow, contractile force and decrease in infarct size. Thus this may lead to a novel cardioprotectant strategy.
本研究探索了印度草药积雪草作为一种心脏保护剂，以心功能和冠状动脉血流为终点指标，对抗缺血/再灌注损伤。
在正常离体大鼠心脏中，使用Langendorff装置测量冠状动脉血流、左心室舒张末压、心率和功能恢复情况。心脏分别用以下溶液灌注：（i）克雷布斯-亨塞尔特（正常）溶液（对照），或30、100μg/ml积雪草乙醇提取物（30分钟）；或（ii）在无灌注缺血（30分钟）前用正常溶液或提取物灌注10分钟，随后用正常溶液再灌注（30分钟）。通过三苯基四氮唑染色测量梗死体积。在小鼠心房心肌细胞系HL-1细胞中，通过全细胞膜片钳记录L型钙电流（I）。通过台盼蓝排斥法评估积雪草对大鼠离体心室肌细胞的细胞毒性。
在正常灌注的心脏中，与对照组（5±3%）相比，积雪草使冠状动脉血流分别增加了63±13%（30μg/ml）和216±21%（100μg/ml）（n = 8 - 10，p < 0.001）。在缺血/再灌注前用积雪草处理，与缺血/再灌注前相比，左心室舒张末压分别提高了84±10%（30μg/ml）、82±10%（100μg/ml）和52±6%（对照组）。同样，功能恢复也持续增加。此外，积雪草（100μg/ml）使梗死面积百分比从51±2%（对照组）降至25±2%（n = 6 - 8，p < 0.0001）。积雪草（100μg/ml）使HL-1细胞中的I降低了63±4%。在较高浓度（50 - 1000μg/ml）的积雪草作用下，心室肌细胞存活率降低。
积雪草通过恢复冠状动脉血流、收缩力并减小梗死面积，改善缺血/再灌注损伤后的心肌功能。因此，这可能会带来一种新的心脏保护策略。
