Beyer W, Möhring R, Drescher B, Nötzel U, Rosenthal S
Department of Cell Differentiation, Academy of Sciences of the German Democratic Republic, Berlin.
Arch Virol. 1987;96(3-4):297-301. doi: 10.1007/BF01320971.
A lambda L 47.1 clone library was constructed from BamHI digests of cellular cat DNA and was screened with cDNA probes specific for the 3'-terminus of the replication competent RD-114 virus. The first analysis of one clone (lambda-ECE 1) shows that it consists at least of a 3'-LTR and a 5' adjacent env gene. The LTR nucleotide sequences of the RD-114 cDNA clones and ECE 1 are identical within the R region and unrelated to FeLV. Otherwise, the region upstream from the 3'-LTR of ECE 1 shows homology to the transmembrane protein encoding gene of FeLV, but not to RD-114. These results imply that ECE 1 is a recombinant between an RD-114-like 3'-LTR and a FeLV-related env portion.
从细胞猫DNA的BamHI酶切片段构建了一个λL 47.1克隆文库，并用针对复制能力强的RD - 114病毒3'末端的cDNA探针进行筛选。对一个克隆（λ - ECE 1）的首次分析表明，它至少由一个3'-LTR和一个5'相邻的env基因组成。RD - 114 cDNA克隆和ECE 1的LTR核苷酸序列在R区域内相同，且与猫白血病病毒（FeLV）无关。否则，ECE 1的3'-LTR上游区域与FeLV的跨膜蛋白编码基因具有同源性，但与RD - 114无关。这些结果表明ECE 1是一个类似RD - 114的3'-LTR与一个FeLV相关的env部分之间的重组体。
