大鼠红细胞中的果糖-2,6-二磷酸。甘油酸-2,3-二磷酸对果糖-2,6-二磷酸合成的抑制作用及测定
Fructose 2,6-bisphosphate in rat erythrocytes. Inhibition of fructose 2,6-bisphosphate synthesis and measurement by glycerate 2,3-bisphosphate.
作者信息
Sobrino F, Rider M H, Gualberto A, Hue L
机构信息
Department of Biochemistry, Faculty of Medicine, University of Sevilla, Spain.
出版信息
Biochem J. 1987 May 15;244(1):235-8. doi: 10.1042/bj2440235.
Abstract
The concentration of fructose 2,6-bisphosphate found in freshly isolated erythrocytes was below the limit of detection (20 pmol/ml of packed cells). However, it increased to about 250 pmol/ml of cells when erythrocytes were incubated with glucose at pH 6.9, but not at pH 7.4 or 8.2. This could be explained by variations in the content of glycerate 2,3-bisphosphate, which was found to inhibit 6-phosphofructo-2-kinase, the enzyme responsible for fructose 2,6-bisphosphate synthesis. Glycerate 2,3-bisphosphate was also found to inhibit the potato enzyme (pyrophosphate:fructose-6-phosphate 1-phosphotransferase) used for the measurement of fructose 2,6-bisphosphate.
摘要
在新鲜分离的红细胞中发现的果糖-2,6-二磷酸浓度低于检测限（每毫升压积细胞20皮摩尔）。然而，当红细胞在pH 6.9条件下与葡萄糖孵育时，其浓度增加至约每毫升细胞250皮摩尔，而在pH 7.4或8.2条件下则不会增加。这可以用2,3-二磷酸甘油酸含量的变化来解释，已发现2,3-二磷酸甘油酸可抑制6-磷酸果糖-2-激酶，该酶负责果糖-2,6-二磷酸的合成。还发现2,3-二磷酸甘油酸可抑制用于测量果糖-2,6-二磷酸的马铃薯酶（焦磷酸：果糖-6-磷酸1-磷酸转移酶）。
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