可可链霉菌和白色链霉菌G的β-内酰胺酶的活性位点。
The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.
作者信息
De Meester F, Joris B, Lenzini M V, Dehottay P, Erpicium T, Dusart J, Klein D, Ghuysen J M, Frère J M, Van Beeumen J
机构信息
Laboratoires de Microbiologie et d'Enzymologie, Université de Liège, Belgium.
出版信息
Biochem J. 1987 Jun 1;244(2):427-32. doi: 10.1042/bj2440427.
Abstract
The active-site serine of the extracellular beta-lactamases of Streptomyces cacaoi and Streptomyces albus G has been labelled with beta-iodopenicillanate. The determination of the sequence of the labelled peptides obtained after trypsin digestion of the denatured proteins indicate both enzymes to be class A beta-lactamases. Surprisingly the two Streptomyces enzymes do not appear to be especially homologous, and none of them exhibited a high degree of homology with the Streptomyces R61 DD-peptidase. Our data confirm that, as a family of homologous enzymes, class A is rather heterogeneous, with only a small number of conserved residues in all members of the class.
摘要
可可链霉菌和白色链霉菌G的细胞外β-内酰胺酶的活性位点丝氨酸已用β-碘青霉素酸盐标记。对变性蛋白质进行胰蛋白酶消化后得到的标记肽段序列测定表明这两种酶均为A类β-内酰胺酶。令人惊讶的是，这两种链霉菌酶似乎并非特别同源，且它们与链霉菌R61 DD-肽酶均未表现出高度同源性。我们的数据证实，作为一个同源酶家族，A类相当异质，该类所有成员中只有少数保守残基。
相似文献
1
The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.可可链霉菌和白色链霉菌G的β-内酰胺酶的活性位点。
Biochem J. 1987 Jun 1;244(2):427-32. doi: 10.1042/bj2440427.
2
Primary structure of the Streptomyces R61 extracellular DD-peptidase. 2. Amino acid sequence data.
Eur J Biochem. 1987 Feb 2;162(3):519-24. doi: 10.1111/j.1432-1033.1987.tb10670.x.
3
Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.链霉菌R61细胞外DD-肽酶的一级结构。1. 克隆到变铅青链霉菌中及该基因的核苷酸序列。
Eur J Biochem. 1987 Feb 2;162(3):509-18. doi: 10.1111/j.1432-1033.1987.tb10669.x.
4
The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family.作为链霉菌R61 DD-肽酶家族成员的活性位点丝氨酸青霉素识别酶。
Biochem J. 1988 Mar 1;250(2):313-24. doi: 10.1042/bj2500313.
5
The beta-lactamase of Streptomyces cacaoi: interaction with cefoxitin and beta-iodopenicillanate.可可链霉菌的β-内酰胺酶：与头孢西丁和β-碘青霉素酸盐的相互作用
J Enzyme Inhib. 1985;1(1):25-34. doi: 10.3109/14756368509031279.
6
Site-directed mutagenesis of the Streptomyces R61 DD-peptidase. Catalytic function of the conserved residues around the active site and a comparison with class-A and class-C beta-lactamases.
Eur J Biochem. 1992 Jul 1;207(1):97-102. doi: 10.1111/j.1432-1033.1992.tb17025.x.
7
Active-site serine mutants of the Streptomyces albus G beta-lactamase.白色链霉菌Gβ-内酰胺酶的活性位点丝氨酸突变体
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):647-52. doi: 10.1042/bj2770647.
8
Transcriptional induction of Streptomyces cacaoi beta-lactamase by a beta-lactam compound.β-内酰胺化合物对可可链霉菌β-内酰胺酶的转录诱导作用
Mol Microbiol. 1989 Oct;3(10):1425-32. doi: 10.1111/j.1365-2958.1989.tb00125.x.
9
Interactions between active-site-serine beta-lactamases and mechanism-based inactivators: a kinetic study and an overview.活性位点丝氨酸β-内酰胺酶与基于机制的失活剂之间的相互作用：动力学研究与综述。
Biochem J. 1993 Nov 1;295 ( Pt 3)(Pt 3):705-11. doi: 10.1042/bj2950705.
10
Cloning and amplified expression in Streptomyces lividans of a gene encoding extracellular beta-lactamase from Streptomyces albus G.来自白色链霉菌G的编码细胞外β-内酰胺酶的基因在变铅青链霉菌中的克隆及扩增表达
Gene. 1986;42(1):31-6. doi: 10.1016/0378-1119(86)90147-2.
引用本文的文献
1
Involvement of a Novel Class C Beta-Lactamase in the Transglutaminase Mediated Cross-Linking Cascade of Streptomyces mobaraensis DSM 40847.一种新型C类β-内酰胺酶参与茂原链霉菌DSM 40847转谷氨酰胺酶介导的交联级联反应
PLoS One. 2016 Feb 17;11(2):e0149145. doi: 10.1371/journal.pone.0149145. eCollection 2016.
2
Cloning and sequencing of the gene encoding Toho-2, a class A beta-lactamase preferentially inhibited by tazobactam.编码Toho-2（一种优先被他唑巴坦抑制的A类β-内酰胺酶）的基因的克隆与测序
Antimicrob Agents Chemother. 1998 May;42(5):1181-6. doi: 10.1128/AAC.42.5.1181.
3
Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli.从大肠杆菌中分离出的一种水解头孢噻肟的A类β-内酰胺酶编码基因的克隆与序列分析
Antimicrob Agents Chemother. 1995 Oct;39(10):2269-75. doi: 10.1128/AAC.39.10.2269.
4
The crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.白色链霉菌G的β-内酰胺酶在0.3纳米分辨率下的晶体结构。
Biochem J. 1987 Aug 1;245(3):911-3. doi: 10.1042/bj2450911.
5
Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site.奇异柠檬酸杆菌的染色体编码β-内酰胺酶。与β-碘青霉素酸盐的相互作用及活性位点的标记
Biochem J. 1988 Sep 15;254(3):891-3. doi: 10.1042/bj2540891.
6
High homology between 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium and Pseudomonas strains.黄杆菌属和假单胞菌属菌株的6-氨基己酸环二聚体水解酶之间的高度同源性。
J Bacteriol. 1989 Jun;171(6):3187-91. doi: 10.1128/jb.171.6.3187-3191.1989.
7
Classification of beta-lactamases: groups 1, 2a, 2b, and 2b'.β-内酰胺酶的分类：1组、2a组、2b组和2b'组。
Antimicrob Agents Chemother. 1989 Mar;33(3):264-70. doi: 10.1128/AAC.33.3.264.
8
Unexpected influence of ionic strength on branched-pathway interactions between beta-lactamases and beta-halogenopenicillanates.离子强度对β-内酰胺酶与β-卤代青霉素酸之间分支途径相互作用的意外影响。
Biochem J. 1989 Jan 1;257(1):245-9. doi: 10.1042/bj2570245.
9
Role of the conserved amino acids of the 'SDN' loop (Ser130, Asp131 and Asn132) in a class A beta-lactamase studied by site-directed mutagenesis.通过定点诱变研究“A类β-内酰胺酶中‘SDN’环（Ser130、Asp131和Asn132）保守氨基酸的作用”
Biochem J. 1990 Oct 15;271(2):399-406. doi: 10.1042/bj2710399.
10
Active-site serine mutants of the Streptomyces albus G beta-lactamase.白色链霉菌Gβ-内酰胺酶的活性位点丝氨酸突变体
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):647-52. doi: 10.1042/bj2770647.
本文引用的文献
1
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
2
The exocellular beta-lactamase of Streptomyces albus G. Purification, properties and comparison with the exocellular DD-carboxypeptidase.白色链霉菌G的胞外β-内酰胺酶。纯化、性质及与胞外DD-羧肽酶的比较。
Biochem J. 1981 Jan 1;193(1):75-82. doi: 10.1042/bj1930075.
3
beta-lactamase from Streptomyces cacaoi. Purification and properties.来自可可链霉菌的β-内酰胺酶。纯化及性质
J Biol Chem. 1981 Mar 25;256(6):2649-55.
4
Interaction of beta-iodopenicillanate with the beta-lactamases of Streptomyces albus G and Actinomadura R39.β-碘青霉烷酸与白色链霉菌G和马杜拉放线菌R39的β-内酰胺酶的相互作用
Biochem J. 1982 Dec 1;207(3):437-44. doi: 10.1042/bj2070437.
5
The structure of beta-lactamases.β-内酰胺酶的结构
Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31. doi: 10.1098/rstb.1980.0049.
6
Strategy and tactics in protein chemistry.蛋白质化学中的策略与战术。
Biochem J. 1970 Oct;119(5):805-22. doi: 10.1042/bj1190805f.
7
Sequence of the OXA2 beta-lactamase: comparison with other penicillin-reactive enzymes.OXA2β-内酰胺酶的序列：与其他青霉素反应性酶的比较。
FEBS Lett. 1985 Oct 21;191(1):39-44. doi: 10.1016/0014-5793(85)80989-3.
8
Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.链霉菌R61细胞外DD-肽酶的一级结构。1. 克隆到变铅青链霉菌中及该基因的核苷酸序列。
Eur J Biochem. 1987 Feb 2;162(3):509-18. doi: 10.1111/j.1432-1033.1987.tb10669.x.
9
Properties of a class C beta-lactamase from Serratia marcescens.粘质沙雷氏菌C类β-内酰胺酶的特性
Biochem J. 1986 Nov 1;239(3):581-6. doi: 10.1042/bj2390581.
10
Structural and kinetic studies on beta-lactamase K1 from Klebsiella aerogenes.产气克雷伯菌β-内酰胺酶K1的结构与动力学研究
Biochem J. 1986 Mar 1;234(2):343-7. doi: 10.1042/bj2340343.
Zotero 插件