粘质沙雷氏菌C类β-内酰胺酶的特性
Properties of a class C beta-lactamase from Serratia marcescens.
作者信息
Joris B, De Meester F, Galleni M, Masson S, Dusart J, Frère J M, Van Beeumen J, Bush K, Sykes R
出版信息
Biochem J. 1986 Nov 1;239(3):581-6. doi: 10.1042/bj2390581.
Abstract
A beta-lactamase produced by a penicillin-resistant strain of Serratia marcescens was isolated and purified. The kcat. value for benzylpenicillin was about 5% of that observed for the best cephalosporin substrates. However, the low Km of the penam resulted in a high catalytic efficiency (kcat./Km) and the classification of the enzyme as a cephalosporinase might not be completely justified. It also exhibited a low but measurable activity against cefotaxime, cefuroxime, cefoxitin and moxalactam. Substrate-induced inactivation was observed both with a very good (cephalothin) or a very bad (moxalactam) substrate. The active site was labelled by beta-iodopenicillanate. Trypsin digestion produced a 19-residue active-site peptide whose sequence clearly allowed the classification of the enzyme as a class C beta-lactamase.
摘要
从一株耐青霉素的粘质沙雷氏菌中分离并纯化出一种β-内酰胺酶。该酶对苄青霉素的kcat.值约为对最佳头孢菌素底物所观察到的值的5%。然而，青霉烷的低Km值导致了高催化效率（kcat./Km），将该酶归类为头孢菌素酶可能并不完全合理。它对头孢噻肟、头孢呋辛、头孢西丁和莫拉卡坦也表现出低但可测量的活性。在非常好的底物（头孢噻吩）或非常差的底物（莫拉卡坦）上均观察到底物诱导的失活。活性位点用β-碘青霉素酸进行标记。胰蛋白酶消化产生了一个19个残基的活性位点肽段，其序列清楚地表明该酶可归类为C类β-内酰胺酶。
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1
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Biochem J. 1982 Feb 1;201(2):425-7. doi: 10.1042/bj2010425.
2
The complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G.白色链霉菌G含锌D-丙氨酰-D-丙氨酸裂解羧肽酶的完整氨基酸序列
Eur J Biochem. 1983 Jan 17;130(1):53-69. doi: 10.1111/j.1432-1033.1983.tb07116.x.
3
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4
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7
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8
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Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31. doi: 10.1098/rstb.1980.0049.
9
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J Biol Chem. 1969 Aug 25;244(16):4406-12.
10
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Jpn J Microbiol. 1968 Dec;12(4):423-34. doi: 10.1111/j.1348-0421.1968.tb00415.x.
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