Ijaz Umer Zeeshan, Quince Christopher, Hanske Laura, Loman Nick, Calus Szymon T, Bertz Martin, Edwards Christine A, Gaya Daniel R, Hansen Richard, McGrogan Paraic, Russell Richard K, Gerasimidis Konstantinos
School of Engineering, University of Glasgow, Glasgow, United Kingdom.
Warwick Medical School, University of Warwick, Warwick, United Kingdom.
PLoS One. 2017 Feb 21;12(2):e0172605. doi: 10.1371/journal.pone.0172605. eCollection 2017.
BACKGROUND/AIMS: Studying the gut microbiota in unaffected relatives of people with Crohn's disease (CD) may advance our understanding of the role of bacteria in disease aetiology.
Faecal microbiota composition (16S rRNA gene sequencing), genetic functional capacity (shotgun metagenomics) and faecal short chain fatty acids (SCFA) were compared in unaffected adult relatives of CD children (CDR, n = 17) and adult healthy controls, unrelated to CD patients (HUC, n = 14). The microbiota characteristics of 19 CD children were used as a benchmark of CD 'dysbiosis'.
The CDR microbiota was less diverse (p = 0.044) than that of the HUC group. Local contribution of β-diversity analysis showed no difference in community structure between the CDR and HUC groups. Twenty one of 1,243 (1.8%) operational taxonomic units discriminated CDR from HUC. The metagenomic functional capacity (p = 0.207) and SCFA concentration or pattern were similar between CDR and HUC (p>0.05 for all SCFA). None of the KEGG metabolic pathways were different between these two groups. Both of these groups (HUC and CDR) had a higher microbiota α-diversity (CDR, p = 0.026 and HUC, p<0.001) with a community structure (β-diversity) distinct from that of children with CD.
While some alterations were observed, a distinct microbial 'dysbiosis', characteristic of CD patients, was not observed in their unaffected, genetically linked kindred.
背景/目的：研究克罗恩病（CD）患者未患病亲属的肠道微生物群，可能会增进我们对细菌在疾病病因学中作用的理解。
对CD患儿的未患病成年亲属（CDR，n = 17）和与CD患者无亲缘关系的成年健康对照者（HUC，n = 14）的粪便微生物群组成（16S rRNA基因测序）、基因功能能力（鸟枪法宏基因组学）和粪便短链脂肪酸（SCFA）进行了比较。将19名CD患儿的微生物群特征作为CD“生态失调”的基准。
CDR微生物群的多样性低于HUC组（p = 0.044）。β多样性分析的局部贡献显示，CDR组和HUC组之间的群落结构没有差异。在1243个操作分类单元中，有21个（1.8%）可区分CDR和HUC。CDR组和HUC组之间的宏基因组功能能力（p = 0.207）以及SCFA浓度或模式相似（所有SCFA的p>0.05）。这两组之间的KEGG代谢途径均无差异。这两组（HUC和CDR）的微生物群α多样性均较高（CDR，p = 0.026；HUC，p<0.001），且群落结构（β多样性）与CD患儿不同。
虽然观察到了一些改变，但在其未患病的、有遗传关联的亲属中未观察到CD患者特有的明显微生物“生态失调”。
