Horiguchi Yukichi, Goda Tatsuro, Matsumoto Akira, Takeuchi Hiroaki, Yamaoka Shoji, Miyahara Yuji
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan.
Department of Molecular Virology, Graduate School of Medical and Dental Sciences, TMDU, 1-5-45 Yushima, Bunkyo, Tokyo 113-8510, Japan.
Biosens Bioelectron. 2017 Jun 15;92:234-240. doi: 10.1016/j.bios.2017.02.023. Epub 2017 Feb 16.
A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.
一种用于区分甲型人流感病毒和禽流感病毒的系统仍然是预防人类流感大流行的一种备受追捧的工具。甲型流感病毒血凝素（HA）与特定唾液酸（SA）受体（人类中的Neu5Acα(2-6)Gal，鸟类中的Neu5Acα(2-3)Gal）的选择性结合由HA和神经氨酸酶（NA）片段的基因型决定，这使其成为区分甲型人流感病毒和禽流感病毒的关键特征之一。在此，我们展示了使用固定有6'-唾液乳糖（Neu5Acα(2-6)Galβ(1-4)Glc，6SL）的金电极作为生物传感表面直接检测完整的甲型H1N1流感病毒。其灵敏度高于传统的流感病毒免疫层析技术（ICT），且不受基因漂移的限制。通过使用化学修饰电极直接附着完整病毒的无标记检测技术是提供一种简单快速的病毒感染诊断系统的有前景的手段。
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