Brookes R H, Kew M C, Rabson A R
MRC Human Cellular Immunology Unit, University of the Witwatersrand, Johannesburg, South Africa.
Cancer Immunol Immunother. 1987;25(2):149-52. doi: 10.1007/BF00199956.
This study investigated the relation between the production of natural killer cytotoxic factors (NKCF) and the phenomenon of natural killing (NK) activity against target K562 cells. Two different models of defective NK cell activity were employed. In the first instance, cytotoxic activity of mononuclear cells (MN) derived from patients with hepatocellular carcinoma was compared to the ability of these cells to produce NKCF. Although direct cytotoxicity was considerably impaired in these patients, the ability of their MN to produce NKCF when stimulated with K562 cells was found to be normal. In the second model, MN treated with the lysosomotropic drug monensin showed considerably reduced direct cytotoxic activity, although they were capable of producing normal amounts of NKCF when activated by K562 cells. These results therefore indicate that there is no correlation between NK activity and corresponding NKCF release, and suggest that NKCF production and activity is independent of direct NK cytotoxic activity.
本研究调查了自然杀伤细胞细胞毒性因子（NKCF）的产生与针对靶细胞K562的自然杀伤（NK）活性现象之间的关系。采用了两种不同的NK细胞活性缺陷模型。首先，将来自肝细胞癌患者的单核细胞（MN）的细胞毒性活性与其产生NKCF的能力进行比较。尽管这些患者的直接细胞毒性明显受损，但发现他们的MN在用K562细胞刺激时产生NKCF的能力是正常的。在第二个模型中，用溶酶体促渗剂莫能菌素处理的MN显示出直接细胞毒性活性显著降低，尽管它们在被K562细胞激活时能够产生正常量的NKCF。因此，这些结果表明NK活性与相应的NKCF释放之间没有相关性，并表明NKCF的产生和活性独立于直接NK细胞毒性活性。
