Group of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia.
Unit of Vector Ecology, Pasteur Institute of Tunis, Tunis 1002, Tunisia.
Int J Mol Sci. 2023 Nov 9;24(22):16116. doi: 10.3390/ijms242216116.
The gene is the most widespread gene encoding aminoglycoside-modifying enzyme and conferring resistance to tobramycin, streptomycin and kanamycin. The variant gene confers resistance to both aminoglycosides and fluoroquinolones (FQ). A total of 132 isolates, including 91 and 41 , were selected from broiler hens isolates. The gene was amplified using PCR and was subsequently digested with the restriction enzyme to identify . Among these isolates, 31 out of 41 (75.6%) and 1 (0.98%) were positive for the -Ib gene, which was identified as the variant in 10 (32.25%) isolates. This variant was correlated with mutations in gyrA (Thr-86-Ile), as well as resistance to FQs. This study is the first report in Tunisia on strains harboring both the and variants. These genes were present in isolates exhibiting resistance to multiple antibiotics, which restricts the range of available treatments.
该基因是编码氨基糖苷类修饰酶的最广泛的基因，可赋予妥布霉素、链霉素和卡那霉素的耐药性。变异基因赋予对氨基糖苷类和氟喹诺酮类（FQ）的耐药性。从肉鸡鸡群中选择了总共 132 株分离株，包括 91 株和 41 株。使用 PCR 扩增基因，然后用限制酶消化以鉴定。在这些分离株中，41 株中的 31 株（75.6%）和 1 株（0.98%）为阳性 -Ib 基因，其中 10 株（32.25%）鉴定为变体。该变体与 gyrA（Thr-86-Ile）的突变以及对 FQ 的耐药性有关。本研究是突尼斯首次报道携带和变体的菌株。这些基因存在于对多种抗生素表现出耐药性的分离株中，这限制了可用治疗方法的范围。
