细胞毒素相关基因A（CagA）诱导胃上皮细胞分泌白细胞介素-8。
CagA induced interleukin-8 secretion in gastric epithelial cells.
作者信息
Fazeli Zeinab, Alebouyeh Masoud, Rezaei Tavirani Mostafa, Azimirad Masoumeh, Yadegar Abbas
机构信息
Faculty of Paramedical Sciences, Department of Basic Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2016 Dec;9(Suppl1):S42-S46.
AIM
Since, contradictory data have been reported about the effect of diverse variants of virulence factors on IL-8 induction, we aimed to analyze the effect of this diversity on levels of IL-8 secretion in AGS cell line.
BACKGROUND
colonizes the human stomach and induces the activation of inflammatory cytokines, including interleukin (IL)-8, in the gastric mucosa. This induction promotes neutrophil and monocyte recruitment that causes gastric tissue damage.
METHODS
To determine whether different strains of and their CagA variants have possible roles on IL-8 induction, polarized AGS cell line was infected with CagA+ strains carrying different EPIYA motifs (ABCCC and ABC) and CagA- strain for 24 hours. Difference in stimulation of IL-8 was measured by ELISA.
RESULTS
IL-8 secretion was elevated in the treated cells with CagA encoding strains compared with the negative one. Furthermore, a noticeably increased level of IL-8 induction was measured by -EPIYA type ABCCC encoding strain in compare to that carried EPIYA type ABC.
CONCLUSION
Results of this study provide new evidence about different effects of strains and possible roles of their CagA variants on IL-8 induction. It seems that not only carriage of and its expression, but also diversity in EPIYA motif be involved in IL-8 induction in the gastric epithelial cells.
目的
由于关于毒力因子不同变体对白细胞介素-8（IL-8）诱导作用的报道相互矛盾，我们旨在分析这种多样性对AGS细胞系中IL-8分泌水平的影响。
背景
幽门螺杆菌定殖于人类胃部并诱导胃黏膜中包括白细胞介素（IL）-8在内的炎性细胞因子活化。这种诱导促进中性粒细胞和单核细胞募集，进而导致胃组织损伤。
方法
为确定幽门螺杆菌不同菌株及其CagA变体是否对IL-8诱导有潜在作用，将极化的AGS细胞系用携带不同EPIYA基序（ABCCC和ABC）的CagA阳性菌株以及CagA阴性菌株感染24小时。通过酶联免疫吸附测定法（ELISA）检测IL-8刺激的差异。
结果
与阴性菌株相比，用编码CagA的菌株处理的细胞中IL-8分泌增加。此外，与携带EPIYA类型ABC的菌株相比，携带-EPIYA类型ABCCC的菌株诱导的IL-8水平显著升高。
结论
本研究结果为幽门螺杆菌菌株的不同作用及其CagA变体对IL-8诱导的潜在作用提供了新证据。似乎不仅幽门螺杆菌的携带及其表达，而且EPIYA基序的多样性都参与胃上皮细胞中IL-8的诱导。
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