Hosseini-Safa Ahmad, Mohag Hegh Mohammad Ali, Pestechian Nader, Ganji Maryam, Mohammadi Rasoul, Mahmoudi Lamouki Reza, Rostami-Nejad Mohammad
Department of Medical Parasitology and Mycology, School of Public Health, Tehran university of Medical Sciences, Tehran, Iran.
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran.
Gastroenterol Hepatol Bed Bench. 2016 Dec;9(Suppl1):S70-S74.
The present study was aimed to evaluate genotypes isolated from goats using HRM analysis in Isfahan province.
Cystic echincoccosis, so-called hydatidosis, is widespread infection caused by the larval stage of . This is an important zoonotic disease worldwide, especially in the developing countries such as Iran. To date, molecular studies mainly based on the mitochondrial DNA sequences have identified distinct genotypes termed G1-G10 which can differ in some characteristics such as the growth and infectivity to different intermediate hosts or the survival rate in the definitive hosts that are important for the development of control strategies.
From August to December 2014, 1341 goats were investigated and hydatid cysts were collected from the liver and lungs of 43 infected goats in Isfahan province abattoirs, Isfahan, Iran. Total genomic DNA was extracted from each sample, amplified for the presence of polymorphism of mitochondrial gene coding for cytochrome c oxidase subunit 1 (CO1), using high resolution melting curve (HRM) method.
the results of HRM analysis using the sequence of CO1 gene for 43 isolates from goats showed 31, 2 and 10 isolates were identified as G1, G2, and G3 genotypes, respectively.
G1 is the predominant genotype in the isolated goat samples in Isfahan province, and the presence of G2 strain was reported for the first time in goat in Iran.
本研究旨在利用高分辨率熔解曲线分析（HRM）评估从伊朗伊斯法罕省山羊中分离出的基因型。
囊型棘球蚴病，即所谓的包虫病，是由 的幼虫阶段引起的广泛感染。这是一种在全球范围内，尤其是在伊朗等发展中国家的重要人畜共患病。迄今为止，主要基于线粒体DNA序列的分子研究已鉴定出不同的基因型，称为G1 - G10，它们在一些特征上可能存在差异，例如对不同中间宿主的生长和感染性，或在终末宿主中的存活率，这些对于制定控制策略很重要。
2014年8月至12月，对1341只山羊进行了调查，并从伊朗伊斯法罕市伊斯法罕省屠宰场的43只受感染山羊的肝脏和肺中收集了包虫囊肿。从每个样本中提取总基因组DNA，使用高分辨率熔解曲线（HRM）方法扩增编码细胞色素c氧化酶亚基1（CO1）的线粒体基因的多态性。
对43株山羊分离株使用CO1基因序列进行HRM分析的结果显示，分别有31株、2株和10株被鉴定为G1、G2和G3基因型。
G1是伊斯法罕省分离的山羊样本中的主要基因型，并且G2菌株在伊朗山羊中首次被报道。
