Pestechian Nader, Hosseini Safa Ahmad, Tajedini Mohammadhasan, Rostami-Nejad Mohammad, Mousavi Mohammad, Yousofi Hosseinali, Haghjooy Javanmard Shaghayegh
Department of Medical Parasitology and Mycology Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Clinical Biochemistry, School of Pharmacy and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. ; Physiology Research Center, Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Korean J Parasitol. 2014 Aug;52(4):413-8. doi: 10.3347/kjp.2014.52.4.413. Epub 2014 Aug 29.
Hydatid cyst caused by Echinococcus granulosus is one of the most important parasitic diseases around the world and many countries in Asia, including Iran, are involved with this infection. This disease can cause high mortality in humans as well as economic losses in livestock. To date, several molecular methods have been used to determine the genetic diversity of E. granulosus. So far, identification of E. granulosus using real-time PCR fluorescence-based quantitative assays has not been studied worldwide, also in Iran. Therefore, the aim of this study was to investigate the genetic diversity of E. granulosus from center of Iran using real-time PCR method. A total of 71 hydatid cysts were collected from infected sheep, goat, and cattle slaughtered in Isfahan, Iran during 2013. DNA was extracted from protoscolices and/or germinal layers from each individual cyst and used as template to amplify the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) (420 bp). Five cattle isolates out of 71 isolates were sterile and excluded from further investigation. Overall, of 66 isolates, partial sequences of the cox1 gene of E. granulosus indicated the presence of genotypes G1 in 49 isolates (74.2%), G3 in 15 isolates (22.7%), and G6 in 2 isolates (3.0%) in infected intermediate hosts. Sixteen sequences of G1 genotype had microgenetic variants, and they were compared to the original sequence of cox1. However, isolates identified as G3 and G6 genotypes were completely consistent with original sequences. G1 genotype in livestock was the dominant genotype in Isfahan region, Iran.
由细粒棘球绦虫引起的包虫囊肿是世界上最重要的寄生虫病之一，包括伊朗在内的亚洲许多国家都有这种感染。这种疾病可导致人类高死亡率以及家畜的经济损失。迄今为止，已经使用了几种分子方法来确定细粒棘球绦虫的遗传多样性。到目前为止，全球范围内以及在伊朗，尚未研究使用基于实时荧光定量PCR的方法来鉴定细粒棘球绦虫。因此，本研究的目的是使用实时PCR方法研究来自伊朗中部的细粒棘球绦虫的遗传多样性。2013年期间，从伊朗伊斯法罕屠宰的受感染绵羊、山羊和牛身上共收集了71个包虫囊肿。从每个囊肿的原头蚴和/或生发层中提取DNA，并用作模板扩增线粒体细胞色素c氧化酶亚基1基因（cox1）（420 bp）。71个分离株中有5个牛分离株无菌，被排除在进一步研究之外。总体而言，在66个分离株中，细粒棘球绦虫cox1基因的部分序列表明，在受感染中间宿主中，49个分离株（74.2%）存在G1基因型，15个分离株（22.7%）存在G3基因型，2个分离株（3.0%）存在G6基因型。G1基因型的16个序列有微基因变异，并与cox1的原始序列进行了比较。然而，鉴定为G3和G6基因型的分离株与原始序列完全一致。伊朗伊斯法罕地区家畜中的G1基因型是优势基因型。
