Shahdoust Samira, Niyyati Maryam, Haghighi Ali, Azargashb Eznoallah, Khataminejad Mohammad Reza
Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Health and Social Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2016 Dec;9(Suppl1):S75-S79.
The aim of the present study was to determine the prevalence of intestinal parasites and their relation with socio-demographic data in referred individuals to the medical centers in Tonekabon, Mazandaran province, 2015.
Due to the climatic and ecological conditions in Mazandaran province, determination of the status of intestinal parasites among referred individuals to the medical centers of Tonekabon city can help researchers and healthcare services to prevent and/or control of parasitic infection in this region.
This cross sectional study was conducted with randomized sampling in 2015 on 820 stool samples. Stool samples were assessed using direct slide smear with saline and Lugol, formalin-ether concentration, Ziehl-Neelsen and trichrome staining. Polymerase Chain Reaction (PCR) using specific primers was conducted for the samples suspected for and spp. One positive sample in this study was submitted for sequencing.
A total of 444 (54.1%) and 376 (45.9%) were male and female, respectively. Furthermore, 495 (60.4%) and 325 (39.6%) of participants had lived in the urban and rural areas, respectively. Overall, 222 participants (27.1%) were infected with at least one intestinal parasites. Prevalence of pathogenic protozoa ( and spp.) and helminthes parasites was calculated as 3.1 and 1.2%, respectively. The most common intestinal parasites in this area were: 153 (18.7%), 44 (5.4%), 40 (4.9%), 25 (3%), 22 (2.7%), 5 (0.6%), 4 (0.5%), 1 (0.1%) and 1 (0.1%). By sequencing of the positive isolate using Gp60 gene, subtype ΠaA16G2R1 was diagnosed.
Protozoa were more abundant than helminthes and was the most common protozoan pathogen. In this study, no significant association was found between the prevalence and the variables of socio-demographic data. Adequate knowledge and periodic surveillance of the prevalence of parasites and the socio-demographic variables that affect their frequency is important for effective control of parasitic infections.
本研究旨在确定2015年转诊至马赞德兰省通卡本医疗中心的人群中肠道寄生虫的患病率及其与社会人口统计学数据的关系。
由于马赞德兰省的气候和生态条件，确定通卡本市医疗中心转诊人群中的肠道寄生虫状况有助于研究人员和医疗服务机构预防和/或控制该地区的寄生虫感染。
2015年，采用随机抽样法对820份粪便样本进行了这项横断面研究。粪便样本采用生理盐水和卢戈氏碘液直接涂片、福尔马林-乙醚浓缩法、萋-尼氏染色法和三色染色法进行评估。对疑似隐孢子虫属和贾第虫属的样本进行了使用特异性引物的聚合酶链反应（PCR）。本研究中一份阳性样本被送去测序。
分别有444名（54.1%）男性和376名（45.9%）女性。此外，分别有495名（60.4%）和325名（39.6%）参与者居住在城市和农村地区。总体而言，222名参与者（27.1%）感染了至少一种肠道寄生虫。致病性原生动物（隐孢子虫属和贾第虫属）和蠕虫寄生虫的患病率分别计算为3.1%和1.2%。该地区最常见的肠道寄生虫为：蓝氏贾第鞭毛虫153例（18.7%）、微小隐孢子虫44例（5.4%）、溶组织内阿米巴40例（4.9%）、结肠内阿米巴25例（3%）、哈氏内阿米巴22例（2.7%）、布氏嗜碘阿米巴5例（0.6%）、微小内蜒阿米巴4例（0.5%）、人芽囊原虫1例（0.1%）和结肠小袋纤毛虫1例（0.1%）。通过对阳性隐孢子虫分离株使用Gp60基因进行测序，诊断为ⅡaA16G2R1亚型。
原生动物比蠕虫更为常见，蓝氏贾第鞭毛虫是最常见的原生动物病原体。在本研究中，未发现患病率与社会人口统计学数据变量之间存在显著关联。充分了解寄生虫患病率以及影响其感染率的社会人口统计学变量并进行定期监测，对于有效控制寄生虫感染非常重要。
