Évora Patricia Martinez, Sanches Gustavo Seron, Guerrero Felix David, León Adalberto Pérez de, Bechara Gervásio Henrique
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil.
Knipling-Bushland U.S. Livestock Insects Research Laboratory, Veterinary Pest Genomics Center, Agricultural Research Service - ARS, United States Department of Agriculture - USDA, Kerrville, Texas, USA.
Rev Bras Parasitol Vet. 2017 Jan-Mar;26(1):60-66. doi: 10.1590/S1984-29612017005. Epub 2017 Feb 16.
This study evaluated a recombinant aquaporin 1 protein of Rhipicephalus (Boophilus) microplus (RmAQP1) as antigen in a vaccine against R. sanguineus. Five dogs were immunized with RmAQP1 (10 µg) + adjuvant (Montanide) (G1), and five were inoculated with adjuvant only (G2), three times. Twenty-one days after the last immunization, animals of both groups were challenged with R. sanguineus larvae, nymphs and adults, and their biotic potential was compared. Blood samples were collected before each immunization and every 28 days after the last immunization for 10 weeks. Serum antibody titers (IgG) were assessed by ELISA. We observed that: engorgement period of adult females from G1 was 12% shorter than G2; larvae from G1 had 8.7% longer engorgement period than G2 and weighed 7.2% less; nymphs from G1 had 4.5% shorter engorgement period than G2 and weighed 3.6% less; although the antibody titers increased following the second immunization, they rapidly decreased after the third immunization. Results indicated low immunoprotection of RmAQP1 against adult R. sanguineus ticks, and possible efficacy on larvae and nymphs fed on immunized dogs. Further studies should be performed for a full evaluation of the immunoprotection of RmAQP1 against R. sanguineus infestations in dogs.
本研究评估了微小扇头蜱（Rhipicephalus (Boophilus) microplus）的重组水通道蛋白1（RmAQP1）作为抗血红扇头蜱（R. sanguineus）疫苗中的抗原。五只犬用RmAQP1（10微克）+佐剂（Montanide）进行免疫（G1组），五只犬仅接种佐剂（G2组），共接种三次。末次免疫后21天，两组动物均用血红扇头蜱的幼虫、若虫和成虫进行攻毒，并比较它们的繁殖力。在每次免疫前以及末次免疫后每28天采集血样，持续10周。通过酶联免疫吸附测定（ELISA）评估血清抗体滴度（IgG）。我们观察到：G1组成年雌性蜱的饱血期比G2组短12%；G1组幼虫的饱血期比G2组长8.7%，但体重轻7.2%；G1组若虫的饱血期比G2组短4.5%，体重轻3.6%；尽管抗体滴度在第二次免疫后升高，但在第三次免疫后迅速下降。结果表明RmAQP1对成年血红扇头蜱的免疫保护作用较低，对取食免疫犬的幼虫和若虫可能有效。应进一步开展研究，以全面评估RmAQP1对犬感染血红扇头蜱的免疫保护作用。
