Contreras Marinela, Karlsen Marius, Villar Margarita, Olsen Rolf Hetlelid, Leknes Lisa Marie, Furevik Anette, Yttredal Karine Lindmo, Tartor Haitham, Grove Soren, Alberdi Pilar, Brudeseth Bjorn, de la Fuente José
SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain.
Pharmaq AS, P.O. Box 267, Skoyen, N-0213 Oslo, Norway.
Vaccines (Basel). 2020 Jan 19;8(1):32. doi: 10.3390/vaccines8010032.
Infestation with the salmon louse (Copepoda, Caligidae) affects Atlantic salmon ( L.) production in European aquaculture. Furthermore, high levels of salmon lice in farms significantly increase challenge pressure against wild salmon populations. Currently, available control methods for salmon louse have limitations, and vaccination appears as an attractive, environmentally sound strategy. In this study, we addressed one of the main limitations for vaccine development, the identification of candidate protective antigens. Based on recent advances in tick vaccine research, herein, we targeted the salmon louse midgut function and blood digestion for the identification of candidate target proteins for the control of ectoparasite infestations. The results of this translational approach resulted in the identification and subsequent evaluation of the new candidate protective antigens, putative Toll-like receptor 6 (P30), and potassium chloride, and amino acid transporter (P33). Vaccination with these antigens provided protection in Atlantic salmon by reducing adult female (P33) or chalimus II (P30) sea lice infestations. These results support the development of vaccines for the control of sea lice infestations.
感染鲑鱼虱（桡足亚纲，海鲺科）会影响欧洲水产养殖中大西洋鲑（L.）的产量。此外，养殖场中高水平的鲑鱼虱会显著增加对野生鲑鱼种群的挑战压力。目前，现有的鲑鱼虱控制方法存在局限性，而疫苗接种似乎是一种有吸引力的、环境友好的策略。在本研究中，我们解决了疫苗开发的主要限制之一，即候选保护性抗原的鉴定。基于蜱疫苗研究的最新进展，在此我们针对鲑鱼虱的中肠功能和血液消化来鉴定控制体外寄生虫感染的候选靶蛋白。这种转化方法的结果导致了新的候选保护性抗原——假定的Toll样受体6（P30）以及氯化钾和氨基酸转运蛋白（P33）的鉴定及后续评估。用这些抗原进行疫苗接种通过减少成年雌虱（P33）或第二期无节幼体（P30）海虱感染，为大西洋鲑提供了保护。这些结果支持开发用于控制海虱感染的疫苗。
