Farajpour Ramin, Sadigh-Eteghad Saeed, Ahmadian Nahid, Farzipour Mohammad, Mahmoudi Javad, Majdi Alireza
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Med Princ Pract. 2017;26(3):245-250. doi: 10.1159/000464364. Epub 2017 Feb 22.
This study was designed to evaluate whether chronic Rosa canina (RC) extract administration could improve recognition memory and depressive-like behavior in diabetic mice.
Seventy-five male albino mice (25-30 g) were randomly divided into 5 groups (15 in each group). A single intraperitoneal injection of 200 mg/kg streptozotocin (STZ) was administered to the mice to induce diabetes. The control group received normal saline, and the diabetic groups received normal saline or 50, 250, and 500 mg/kg of RC extract for 28 days. The mice were weighed each week. Recognition memory and depressive-like behavior were assessed using forced swimming and novel object recognition (NOR) tests, respectively. Malondialdehyde (MDA) levels and total antioxidant capacity (TAC) were measured in the mouse brain homogenate to evaluate oxidative stress. Statistical analysis was conducted using SPSS, version 22.
The groups receiving 250 or 500 mg/kg RC had significantly lower immobility time (159.4 ± 4.7 and 150.1 ± 3.1 s) compared to the sham control group (192.1 ± 7.8 s) in the forced swimming test, and a higher discrimination index (0.39 ± 0.02 and 0.48 ± 0.03) was seen in diabetic animals in the NOR task compared to the sham control group (0.2 ± 0.01). Also, the groups receiving treatment with RC (250 and 500 mg/kg) had significantly higher TAC (0.92 ± 0.04 and 0.96 ± 0.05 mmol/L) and lower MDA (0.76 ± 0.02 and 0.67 ± 0.03 nmol/mg protein) levels in the brains in comparison to the model group. In the 3rd and 4th weeks of study, the RC-treated mice (250 and 500 mg/kg) gained more weight (31.2 ± 0.3 and 32.4 ± 0.3 g, and 31.3 ± 0.2 and 33.7 ± 0.3 g, respectively) than the diabetic group (30 ± 0.2 and 29.6 ± 0.3 g).
This study showed that RC attenuated impairment of recognition memory and depressive-like behavior probably through modulation of oxidative stress in an STZ model of diabetes in mouse brains.
本研究旨在评估长期给予犬蔷薇（RC）提取物是否能改善糖尿病小鼠的认知记忆和抑郁样行为。
75只雄性白化小鼠（25 - 30克）随机分为5组（每组15只）。给小鼠单次腹腔注射200毫克/千克链脲佐菌素（STZ）以诱导糖尿病。对照组接受生理盐水，糖尿病组接受生理盐水或50、250和500毫克/千克的RC提取物，持续28天。每周称小鼠体重。分别使用强迫游泳和新物体识别（NOR）试验评估认知记忆和抑郁样行为。在小鼠脑匀浆中测量丙二醛（MDA）水平和总抗氧化能力（TAC）以评估氧化应激。使用SPSS 22版进行统计分析。
在强迫游泳试验中，接受250或500毫克/千克RC的组与假手术对照组（192.1±7.8秒）相比，不动时间显著缩短（分别为159.4±4.7和150.1±3.1秒），并且在NOR任务中，糖尿病动物与假手术对照组（0.2±0.01）相比，辨别指数更高（分别为0.39±0.02和0.48±0.03）。此外，与模型组相比，接受RC（250和500毫克/千克）治疗的组大脑中的TAC显著更高（分别为0.92±0.04和0.96±0.05毫摩尔/升），MDA水平更低（分别为0.76±0.02和0.67±0.03纳摩尔/毫克蛋白质）。在研究的第3周和第4周，接受RC治疗的小鼠（250和500毫克/千克）体重增加更多（分别为31.2±0.3和32.4±0.3克，以及31.3±0.2和33.7±0.3克），高于糖尿病组（30±0.2和29.6±0.3克）。
本研究表明，在小鼠脑内的STZ糖尿病模型中，RC可能通过调节氧化应激减轻了认知记忆损害和抑郁样行为。
