Yao Wei, Ruan Miaohong, Qin Lifang, Yang Chuanyu, Chen Rukai, Chen Baoshan, Zhang Muqing
State Key Laboratory of Conservation and Utilization of Subtropical Agri-Biological Resources, Guangxi UniversityNanning, China; Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityFuzhou, China.
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University Fuzhou, China.
Front Plant Sci. 2017 Feb 8;8:104. doi: 10.3389/fpls.2017.00104. eCollection 2017.
Sugarcane mosaic disease is mainly caused by the (SCMV), which can significantly reduce stalk yield and sucrose content of sugarcane in the field. Coat protein mediated protection (CPMP) is an effective strategy to improve virus resistance. A 2-year field study was conducted to compare five independent transgenic sugarcane lines carrying the SCMV-CP gene (i.e., B2, B36, B38, B48, and B51) with the wild-type parental clone Badila (WT). Agronomic performance, resistance to SCMV infection, and transgene stability were evaluated and compared with the wild-type parental clone Badila (WT) at four experimental locations in China across two successive seasons, i.e., plant cane (PC) and 1st ratoon cane (1R). All transgenic lines derived from Badila had significantly greater tons of cane per hectare (TCH) and tons of sucrose per hectare (TSH) as well as lower SCMV disease incidence than those from Badila in the PC and 1R crops. The transgenic line B48 was highly resistant to SCMV with less than 3% incidence of infection. The recovery phenotype of transgenic line B36 was infected soon after virus inoculation, but the subsequent leaves showed no symptoms of infection. Most control plants developed symptoms that persisted and spread throughout the plant with more than 50% incidence. B48 recorded an average of 102.72 t/ha, which was 67.2% more than that for Badila. The expression of the transgene was stable over many generations with vegetative propagation. These results show that SCMV-resistant transgenic lines derived from Badila can provide resistant germplasm for sugarcane breeding and can also be used to study virus resistance mechanisms. This is the first report on the development and field performance of transgenic sugarcane plants that are resistant to SCMV infection in China.
甘蔗花叶病主要由甘蔗花叶病毒（SCMV）引起，在田间可显著降低甘蔗的茎产量和蔗糖含量。外壳蛋白介导的保护作用（CPMP）是提高病毒抗性的有效策略。开展了一项为期两年的田间研究，以比较携带SCMV - CP基因的五个独立转基因甘蔗品系（即B2、B36、B38、B48和B51）与野生型亲本克隆Badila（WT）。在中国的四个试验地点，连续两个季节（即新植蔗（PC）和第一季宿根蔗（1R））对农艺性状表现、对SCMV感染的抗性和转基因稳定性进行了评估，并与野生型亲本克隆Badila（WT）进行了比较。在新植蔗和第一季宿根蔗作物中，所有源自Badila的转基因品系每公顷甘蔗吨数（TCH）和每公顷蔗糖吨数（TSH）均显著高于Badila，且SCMV发病率更低。转基因品系B48对SCMV高度抗性，感染发病率低于3%。转基因品系B36在病毒接种后很快出现恢复表型，但随后的叶片未显示感染症状。大多数对照植株出现持续且蔓延至全株的症状，发病率超过50%。B48平均产量为102.72吨/公顷，比Badila高67.2%。转基因通过营养繁殖在多代中表达稳定。这些结果表明，源自Badila的抗SCMV转基因品系可为甘蔗育种提供抗性种质，也可用于研究病毒抗性机制。这是中国关于抗SCMV感染转基因甘蔗植株的培育及田间表现的首次报道。
