Bedell M A, Jones K H, Laimins L A
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
J Virol. 1987 Nov;61(11):3635-40. doi: 10.1128/JVI.61.11.3635-3640.1987.
Plasmids containing the E6 and E7 open reading frames of human papillomavirus type 18 expressed from an autologous transcriptional control region were sufficient for transformation of NIH 3T3 and Rat-1 cells. Transformation by these sequences did not always involve morphological alterations even though anchorage-independent growth occurred at a high frequency. In these cells, the efficiency of transformation by the E6 and E7 construct was equivalent to or, in most cases, better than that observed with the entire viral genome. These data indicate that a major human papillomavirus type 18 transforming function is localized in either the E6 or E7 gene products or both.
含有从自体转录控制区表达的人乳头瘤病毒18型E6和E7开放阅读框的质粒足以转化NIH 3T3和大鼠1细胞。尽管非锚定依赖性生长频繁发生，但这些序列介导的转化并不总是涉及形态学改变。在这些细胞中，E6和E7构建体的转化效率等同于或在大多数情况下优于用整个病毒基因组观察到的效率。这些数据表明，人乳头瘤病毒18型的主要转化功能定位于E6或E7基因产物或两者中。
