de Almeida Danielle Ayr Tavares, Rosa Suellen Iara Guirra, da Cruz Thais Campos Dias, Pavan Eduarda, Sabino Damazo Amílcar, Soares Ilsamar Mendes, Ascêncio Sérgio Donizeti, Macho Antonio, Martins Domingos Tabajara de Oliveira
Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso, Av. Fernando Correa da Costa, n. 2367, Cuiabá, Mato Grosso 78060-900, Brazil.
Research Laboratory of Natural Products (LPPN), Federal University of Tocantins, Av. NS15, Palmas, Tocantins 77020-210, Brazil.
J Ethnopharmacol. 2017 Mar 22;200:51-59. doi: 10.1016/j.jep.2017.02.015. Epub 2017 Feb 14.
Mandevilla longiflora, popularly known as "velame" in central Brazil, is a subshrub widely distributed in South America. Its xylopodium is used in the form of a decoction or infusion to treat inflammation and other ailments.
This study aimed to evaluate the anti-inflammatory potential of M. longiflora in an in vivo model of ovalbumin-induced immediate hypersensitivity, identifying its effects on leukocyte infiltration, IgE and LTB levels, and Th2 cytokine production. In addition, HPLC fingerprint of the extract was performed.
The hydroethanolic extract 70% of M. longiflora (HEMI) was obtained by maceration of the plant xylopodium. Swiss mice were sensitized by i.p. injection OVA-aluminium hydroxide on days 1 and 10. Nine days after the last sensitisation animals were challenged for 6 consecutive days with OVA solution for 20min daily in a closed chamber under continuous flow of aerosol. The animals were treated with HEMl (20, 50 and 200mg/kg p.o.), 2 times per day, and euthanized 24h later. Animals treated with vehicle (2% Tween-20) or dexamethasone were used as negative and positive controls, respectively. The recruitment of inflammatory cells into the pulmonary cavity was evaluated by counting cells present in broncho-alveolar lavage fluid (BALF). Lung tissue was also collected for histopathology and infiltration analysis. Quantification of IL-4, IL-5 and IL-13 from the BALF, and IgE, and LTB from plasma, were conducted by immunoassay.
The HEMl attenuated leukocyte migration into the airways, which was evidenced by a decrease in eosinophils, neutrophils and mononuclear cells, both in BALF quantification and by histopathological analysis, as well as decreasing the concentrations of IL-4, IL-5, IL-13, IgE and LTB. All of these events are typical of air-mucosa inflammatory disease. These findings scientifically evidence for the first time the ethnopharmacological use of M. longiflora to treat chronic inflammatory events, such as asthma, and suggest a potential therapeutic use or complementary therapy for this plant extract.
长花曼德维拉，在巴西中部俗称“velame”，是一种广泛分布于南美洲的亚灌木。其根状茎以煎剂或浸剂的形式用于治疗炎症和其他疾病。
本研究旨在评估长花曼德维拉在卵清蛋白诱导的速发型超敏反应体内模型中的抗炎潜力，确定其对白细胞浸润、IgE和白三烯B水平以及Th2细胞因子产生的影响。此外，还对提取物进行了高效液相色谱指纹图谱分析。
通过浸渍植物根状茎获得70%的长花曼德维拉水乙醇提取物（HEMI）。在第1天和第10天，通过腹腔注射卵清蛋白-氢氧化铝使瑞士小鼠致敏。在最后一次致敏后9天，动物在封闭室内，在持续气溶胶流的条件下，每天用卵清蛋白溶液攻击20分钟，连续6天。动物每天口服HEMI（20、50和200mg/kg）2次，并在24小时后安乐死。分别用赋形剂（2%吐温-20）或地塞米松处理的动物作为阴性和阳性对照。通过计数支气管肺泡灌洗液（BALF）中的细胞来评估炎症细胞向肺腔的募集。还收集肺组织进行组织病理学和浸润分析。通过免疫测定法对BALF中的IL-4、IL-5和IL-13以及血浆中的IgE和白三烯B进行定量。
HEMI减轻了白细胞向气道的迁移，这在BALF定量和组织病理学分析中均表现为嗜酸性粒细胞、中性粒细胞和单核细胞数量减少，同时降低了IL-4、IL-5、IL-13、IgE和白三烯B的浓度。所有这些事件都是气道-黏膜炎症性疾病的典型表现。这些发现首次科学地证明了长花曼德维拉在治疗慢性炎症性疾病（如哮喘）方面的民族药理学用途，并表明这种植物提取物具有潜在的治疗用途或辅助治疗作用。
