Williamson V M, Paquin C E
ARCO Plant Cell Research Institute, Dublin, CA 94568.
Mol Gen Genet. 1987 Sep;209(2):374-81. doi: 10.1007/BF00329668.
Insertion of the transposable element Ty at the ADH4 locus results in increased levels of a new alcohol dehydrogenase (ADH) activity in Saccharomyces cerevisiae. The DNA sequence of this locus has been determined. It contains a long open reading frame which is not homologous to the other ADH isozymes that have been characterized in S. cerevisiae nor does it show obvious homology to Drosophila ADH. The hypothetical ADH does, however, show strong homology to the sequence of an iron-activated ADH from the bacterium Zymomonas mobilis. Thus ADH4 appears to encode an ADH structural gene which, along with the Zymomonas enzyme, may define a new family of alcohol dehydrogenases.
转座因子Ty插入酿酒酵母的ADH4基因座会导致一种新的乙醇脱氢酶（ADH）活性水平升高。已确定该基因座的DNA序列。它包含一个长的开放阅读框，该开放阅读框与酿酒酵母中已鉴定的其他ADH同工酶不同源，也与果蝇ADH没有明显的同源性。然而，推测的ADH与运动发酵单胞菌的铁激活ADH序列具有很强的同源性。因此，ADH4似乎编码一个ADH结构基因，该基因与运动发酵单胞菌的酶一起可能定义了一个新的乙醇脱氢酶家族。
