Daneshpour Maryam S, Fallah Mohammad-Sadegh, Sedaghati-Khayat Bahareh, Guity Kamran, Khalili Davood, Hedayati Mehdi, Ebrahimi Ahmad, Hajsheikholeslami Farhad, Mirmiran Parvin, Ramezani Tehrani Fahimeh, Momenan Amir-Abbas, Ghanbarian Arash, Amouzegar Atieh, Amiri Parisa, Azizi Fereidoun
Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic Of Iran.
Prevention Of Metabolic Disorders Research Center, Research Institute For Endocrine Sciences, Shahid Beheshti University Of Medical Sciences, Tehran, Islamic Republic Of Iran.
JMIR Res Protoc. 2017 Feb 23;6(2):e28. doi: 10.2196/resprot.6050.
Cardiometabolic risk factors comprise cardiovascular diseases and/or diabetes, and need to be evaluated in different fields.
The primary aim of the Tehran Cardiometabolic Genetic Study (TCGS) is to create a comprehensive genome-wide database of at least 16,000 Tehranians, who are participants of the ongoing Tehran Lipid and Glucose Study (TLGS) cohort.
TCGS was designed in collaboration with the Research Institute for Endocrine Sciences and the genetic company deCODE. Participants had already been followed for over a 20-year period for major cardiometabolic-related health events including myocardial infarction, stroke, diabetes mellitus, hypertension, obesity, hyperlipidemia, and familial hypercholesterolemia.
The TCGS cohort described here comprises 17,186 (86.3%) of the 19,905 TLGS participants who provided a baseline blood sample that was adequate for plasma and deoxyribonucleic acid analysis. This study is comprised of 849 individuals and 3109 families with at least one member having genotype information. Finally, 5977 males and 7422 females with the total genotyping rate of 0.9854 were genotyped with HumanOmniExpress-24-v1-0 bead chips (containing 649,932 single-nucleotide polymorphism loci with an average mean distance of 4 kilobases).
Investigations conducted within the TCGS will seek to identify relevant patterns of genetic polymorphisms that could be related to cardiometabolic risk factors in participants from Tehran. By linking genome-wide data to the existing databank of TLGS participants, which includes comprehensive behavioral, biochemical, and clinical data on each participant since cohort inception in 1999, the TCGS will also allow exploration of gene-gene and gene-environment interactions as they relate to disease status.
心脏代谢危险因素包括心血管疾病和/或糖尿病，需要在不同领域进行评估。
德黑兰心脏代谢基因研究（TCGS）的主要目的是创建一个至少包含16000名德黑兰人的全基因组数据库，这些人是正在进行的德黑兰脂质与葡萄糖研究（TLGS）队列的参与者。
TCGS是与内分泌科学研究所和基因公司deCODE合作设计的。参与者已经被跟踪了20多年，以观察主要的心脏代谢相关健康事件，包括心肌梗死、中风、糖尿病、高血压、肥胖、高脂血症和家族性高胆固醇血症。
这里描述的TCGS队列包括19905名TLGS参与者中的17186名（86.3%），他们提供了足以进行血浆和脱氧核糖核酸分析的基线血样。本研究由849名个体和3109个家庭组成，其中至少有一名成员具有基因型信息。最后，使用HumanOmniExpress-24-v1-0珠芯片（包含649932个单核苷酸多态性位点，平均间距为4千碱基）对5977名男性和7422名女性进行基因分型，总基因分型率为0.9854。
在TCGS内进行的研究将试图识别可能与德黑兰参与者心脏代谢危险因素相关的遗传多态性相关模式。通过将全基因组数据与TLGS参与者的现有数据库相链接，该数据库包括自1999年队列开始以来每个参与者的全面行为、生化和临床数据，TCGS还将允许探索与疾病状态相关的基因-基因和基因-环境相互作用。
