KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven Dentistry, Kapucijnenvoer 7, Blok A-Box 7001, BE-3000 Leuven, Belgium; Wuhan University, School and Hospital of Stomatology, Ministry of Education, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine, Wuhan, PR China.
KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven Dentistry, Kapucijnenvoer 7, Blok A-Box 7001, BE-3000 Leuven, Belgium.
Dent Mater. 2017 Apr;33(4):434-445. doi: 10.1016/j.dental.2017.01.015. Epub 2017 Feb 21.
To characterize the chemical interplay and to quantify the re-mineralization potential of hydraulic calcium-silicate cements (hCSCs) at demineralized dentin.
Pairs of class-I cavities were prepared in non-carious human third molars. One dentin cavity was demineralized with 10% formic acid (5h); the other served as control. The cavities were filled with two resin-free hCSCs (Biodentine, Septodont; ProRoot MTA, Dentsply Sirona) or one resin-based hCSC (TheraCal LC, Bisco). After 1-week, 1-, 3-, and 6-month storage in simulated body fluid (SBF), polished specimen cross-sections were chemically characterized using Field-emission-gun Electron Probe Micro-Analysis (Feg-EPMA) and micro-Raman spectroscopy (μRaman).
Feg-EPMA line-scans and elemental mappings confirmed early re-mineralization induced by all three hCSCs at 1week. The relative depth and intensity of re-mineralization were for the resin-free hCSCs in the range of 50.5%-84.8% and 68.1%-89.2%, respectively. Re-mineralization did not significantly differ for the storage periods (p>0.05). Significantly less re-mineralization was achieved by the resin-based hCSC TheraCal LC that reached only at 6months a re-mineralization level that was no longer significantly different from that achieved by the resin-free hCSCs at 1week (p>0.05). Re-mineralization of intertubular dentin, including tubular occlusion, was observed; Si was occasionally detected to have infiltrated the dentin tubules. Dentin re-mineralization by hCSCs was confirmed using μRaman that revealed an increased phosphate peak at 960cm.
hCSCs do re-mineralize demineralized dentin. The resin-free cements induced re-mineralization at a higher speed/intensity than the resin-based hCSC. However, re-mineralization was incomplete for all hCSCs tested, this even at 6months.
研究水合硅酸钙水泥（hCSCs）在脱矿牙本质中的化学相互作用，并定量其再矿化潜力。
在非龋性第三磨牙上制备 I 类洞。一个牙本质洞用 10%甲酸（5h）脱矿；另一个作为对照。用两种无树脂 hCSCs（Biodentine，Septodont；ProRoot MTA，Dentsply Sirona）或一种树脂基 hCSC（TheraCal LC，Bisco）填充牙洞。在模拟体液（SBF）中储存 1 周、1 个月、3 个月和 6 个月后，使用场发射枪电子探针微分析（Feg-EPMA）和微拉曼光谱（μRaman）对抛光标本的横截面进行化学表征。
Feg-EPMA 线扫描和元素映射证实了所有三种 hCSC 在 1 周时早期诱导的再矿化。无树脂 hCSCs 的相对深度和再矿化强度分别为 50.5%-84.8%和 68.1%-89.2%。不同储存时间（p>0.05）之间的再矿化没有显著差异。树脂基 hCSC TheraCal LC 的再矿化程度明显较低，仅在 6 个月时达到与无树脂 hCSC 在 1 周时相似的再矿化水平（p>0.05）。观察到管间牙本质的再矿化，包括管腔闭塞，偶尔检测到 Si 渗入牙本质小管。hCSCs 使牙本质再矿化，μRaman 显示 960cm 处磷酸盐峰增加。
hCSCs 确实使脱矿牙本质再矿化。无树脂水泥诱导再矿化的速度/强度高于树脂基 hCSC。然而，所有测试的 hCSCs 都没有完全再矿化，即使在 6 个月时也是如此。
