使用具有仿生模拟物的实验硅酸三钙水泥使牙本质再矿化。
Re-mineralizing dentin using an experimental tricalcium silicate cement with biomimetic analogs.
机构信息
KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven Dentistry, Kapucijnenvoer 7, blok A-box 7001, BE-3000 Leuven, Belgium; Wuhan University, School and Hospital of Stomatology, Ministry of Education, The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine, Wuhan, PR China.
KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT & University Hospitals Leuven Dentistry, Kapucijnenvoer 7, blok A-box 7001, BE-3000 Leuven, Belgium.
出版信息
Dent Mater. 2017 May;33(5):505-513. doi: 10.1016/j.dental.2017.02.003. Epub 2017 Mar 6.
OBJECTIVES
To characterize the re-mineralization potential of an experimental zirconium oxide (ZrO) containing tricalcium silicate (TCS) cement, TCS 50, with the incorporation of biomimetic analogs at demineralized dentin.
METHODS
Class-I cavities were prepared in non- carious human third molars. The dentin cavities were demineralized using a pH-cycling protocol, involving 50 cyclic immersions in pH-4.8 and pH-7 baths for 0.5h and 2.5h, successively. The cavities were filled with TCS 50 with/without biomimetic analogs (3% polyacrylic acid, 8% sodium trimetaphosphate) being added to the mixed TCS 50 cement prior to application. The commercial hCSCs Biodentine (Septodont) and ProRoot MTA (Dentsply Sirona) served as controls. After 1 and 6 weeks storage in simulated body fluid (SBF), the polished specimen cross-sections were chemically characterized using a field-emission-gun Electron Probe Micro-Analysis (Feg- EPMA).
RESULTS
EPMA line-scans and elemental mappings confirmed early re-mineralization induced by TCS 50 at 1 week. When biomimetic analogs were added to TCS 50, re-mineralization was more efficient after 6 weeks; the relative depth and intensity of re-mineralization were 79.7% and 76.6%, respectively, being significantly greater than at 1 week (pSignificance: The experimental TCS-based cement, TCS 50, proved to be capable of re-mineralizing artificially demineralized dentin. The incorporation of biomimetic analogs promoted re- mineralization upon 6-week SBF storage. However, re-mineralization appeared incomplete, this even for TCS 50 to which biomimetic analogs were added and upon 6-week SBF storage.
目的
研究一种含有硅酸三钙（TCS）的氧化锆（ZrO）实验性水泥（TCS 50）在脱矿牙本质中仿生类似物的加入对再矿化潜力的影响。
方法
在非龋性第三磨牙上制备 I 类窝洞。使用 pH 循环方案使牙本质窝洞脱矿，包括在 pH 4.8 和 pH 7 的浴中进行 50 次 0.5h 和 2.5h 的循环浸泡。将 TCS 50 与/或不添加仿生类似物（3%聚丙烯酸、8%偏磷酸三钠）的混合物填入 TCS 50 水泥中，然后进行应用。商用 hCSCs Biodentine（Septodont）和 ProRoot MTA（Dentsply Sirona）作为对照。在模拟体液（SBF）中储存 1 周和 6 周后，使用场发射枪电子探针微分析（Feg-EPMA）对抛光标本的横截面进行化学表征。
结果
EPMA 线扫描和元素映射证实，TCS 50 在 1 周时即可诱导早期再矿化。当仿生类似物添加到 TCS 50 中时，6 周后的再矿化效率更高；再矿化的相对深度和强度分别为 79.7%和 76.6%，显著高于 1 周时（p<0.05）。与 Biodentine 和 ProRoot MTA 相比，TCS 50 水泥在储存 1 周和 6 周后，其元素钙和磷含量均高于对照组（p<0.05）。
结论
实验性 TCS 基水泥 TCS 50 被证明能够再矿化人工脱矿的牙本质。在 SBF 储存 6 周时，添加仿生类似物可促进再矿化。然而，即使添加了仿生类似物，TCS 50 经 6 周 SBF 储存后的再矿化似乎仍不完全。
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